Correlation between intestinal flora diversity before treatment and the efficacy of probiotics in patients with irritable bowel syndrome
Objective To analyze the changes in gut microbiota and efficacy scores in patients with irritable bowel syndrome(IBS)after probiotic treatment,and to explore the correlation between the gut microbiota characteristics of IBS patients before treatment and the effectiveness of probiotic therapy.Methods A total of 50 patients with diarrhea-predominant irritable bowel syndrome(IBS-D)treated at the Xinjiang Military Region General Hospital from Mar 2021 to Mar 2022 were included.Probiotics(Bifidobacterium and Lactobacillus triple viable tablets)were administered for 4 weeks on the basis of routine treatment.Fecal samples were collected before and after treatment,and the overall struc-ture and diversity characteristics of the gut microbiota were analyzed using 16S rDNA sequencing,comparing significant-ly different species.Changes in IBS quality of life(IBS-QOL),IBS symptom severity scale(IBS-SSS),gastrointestinal symptoms rating scales(GSRS),and fecal type scale(Bristol stool form scale,BSFS)scores before and after treatment were compared.Pearson correlation analysis was used to analyze the correlation between the levels of gut microbiota in IBS patients and the scores of various scales.Results In terms of gut microbiota α-diversity,the Chao and Ace indices before treatment were lower than after treatment,with statistically significant differences(P<0.05).There were obvious statistical differences in the β-diversity of the gut microbiota before and after treatment(P<0.05).Before treatment,Enterobacteriaceae,Streptococcus and Lactobacillus were dominant in the intestinal flora of patients;Bacteroidetes and Bifidobacterium were dominant after treatment.In terms of clinical efficacy,the IBS-QOL score after treatment in the ex-perimental group was higher than before treatment,while the IBS-SSS score,GSRS score and BSFS score were lower than before treatment,with statistically significant differences(P<0.05).Pearson correlation analysis showed that the levels of Clostridium,Actinobacteria,Streptococcus,Fecal bacilli,and Lactobacillus were negatively correlated with IBS-QOL scores,but positively correlated with IBS-SSS scores,GSRS scores and BSFS scores(P<0.05).Conclusion Early application of probiotics can effectively improve the clinical symptoms of IBS patients,and there is a certain correlation between the level of gut microbiota in IBS patients and the effectiveness of probiotic therapy.
Gut microbiotaIrritable bowel syndromeProbioticsRelativity
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NSTL
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