Analysis of the relationship between chemoresistance and gut microbiota in colorectal cancer patients based on metagenomics
Objective To investigate the relationship between chemoresistance and gut microbiota in colorectal cancer patients based on metagenomics.Methods A total of 150 colorectal cancer patients admitted to the hospital from Sep.2021 to Mar.2023 were selected as the research subjects,and another 150 healthy individuals were selected as the healthy group.According to the chemotherapy sensitivity of colorectal cancer patients,150 patients were divided into a sensitive group(n=82)and an insensitive group(n=68).The abundance of OTU microbiota,α-diversity,β-diversity,differential strains and differential resistance genes in both groups were analyzed through metagenomic sequencing.The correlation between the changes of gut microbiota and drug resistance genes was determined by correlation analysis.Results The abundance of OTU microbiota,α-diversity,β-diversity in the colorectal cancer group was lower than that of the healthy group.The abundance of OTU microbiota,α-diversity,(3-diversity in the insensitive group was lower than that of the sensitive group.There were a total of 24 differential bacterial strains between the colorectal cancer group and the healthy group,of which 6 strains were significantly enriched in the intestines of colorectal cancer patients,and 18 strains were significantly reduced in the colorectal cancer group.Compared with the healthy group,the insensitive group showed a significant enrichment of 6 bacterial strains.There was a significant difference between the colorectal cancer group and healthy group in the 13 drug resistance genes,and all of them were enriched in the intestine of the colorectal cancer group.The enrichment of 13 drug resistance genes in the intestine of the insensitive group was significantly higher than that of the sensitive group.A correlation analysis was conducted between differential gut microbiota and drug resist-ance genes in the colorectal cancer group and the healthy group.Six differential bacterial strains were positively correla-ted with 13 drug resistance genes,respectively.Conclusion The abundance of gut microbiota in colorectal cancer pa-tients is significantly reduced,and some of the flora is closely related to chemotherapy sensitivity,and is significantly positively related to drug resistance genes.Indicating that biomarkers of bacteria and drug resistance genes may be used for colorectal cancer screening and chemotherapy efficacy evaluation.
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