The expression and significance of Smac/DIABLO in liver tissue of rats with non-alcoholic fatty liver disease
Objective To investigate the expression and significance of Smac/DIABLO in the liver tissue of rats with non-alcoholic fatty liver disease(NAFLD).Methods Rats were randomly grouped into a control group and a high-fat group.The control group was fed with basic feed,while the high-fat group was fed with high-fat feed.The changes in body weight and liver index of rats were observed on the 4th,8th and 12th week of feeding,respectively.The kits were applied to detect the blood lipid indicators TG,TC and liver function indicators ALT,AST;HE staining and oil red O staining were applied to observe pathological changes in liver tissue,flow cytometry was applied to detect liver cell apop-tosis,immunohistochemistry was applied to detect the expression of Smac/DIABLO and Caspase-3 proteins,Western blotting was applied to detect the expression of Smac/DIABLO,X-linked apoptosis inhibitory protein(XIAP),Caspase-3 and Caspase-9 proteins,qRT-PCR was applied to detect the expression of Smac/DIABLO,XIAP,Caspase-3 and Caspase-9 genes.Results Compared with the control group,the rats in the high-fat group had disordered arrangement of liver cells at weeks 4,8 and 12,with obvious fat vacuoles,inflammatory cell infiltration,and severe lipid accumula-tion,the body weight,liver index,TC,TG,ALT,AST,NAS score and apoptosis rate obviously increased(P＜0.05);the immunohistochemical results showed that compared to the control group,the expression of Smac/DIABLO and Caspase-3 in the liver tissue of the high-fat group rats increased,the IOD value obviously increased(P＜0.05);West-ern blotting and qRT-PCR results showed that compared to the control group,the expression of Smac/DIABLO,Caspase-3,Caspase-9 proteins and mRNA in the liver tissue of high-fat group rats obviously increased,the expression of XIAP obviously reduced(P＜0.05).Conclusion The expression of Smac/DIABLO protein in liver tissue of NAFLD rats is obviously increased,which is closely related to liver cell apoptosis.
NETL
NSTL
万方数据
