Effects of Bifidobacterium tetragenous viable bacteria tablets on gut microbiota in non-alcoholic fatty liver disease patients based on high-throughput sequencing
Objective To investigate the effect of Bifidobacterium tetragenous viable bacteria tablets on gut microbiota in non-alcoholic fatty liver disease(NAFLD)patients.Methods Twenty patients with NAFLD admitted to our hospital from Jul.2021 to Jan.2022 were enrolled as the NAFLD group,and 20 subjects who came to our hospital for health examination during the same period were enrolled as healthy control group.NAFLD patients were given bifidobacterium tetragenous viable bacteria tablets orally for 4 weeks.Liver function and blood lipid levels were detected,and fecal samples were collected be-fore and after treatment.Fecal samples of NAFLD patients and healthy controls were detected by 16S rDNA sequencing to an-alyze the composition and structure of gut microbiota.Results Bifidobacterium treatment significantly improved the levels of ALT,AST,ALP,TC and HDL in NAFLD patients.Bifidobacterium treatment did not alter the Alpha diversity of NAFLD pa-tients,but the Beta diversity was significantly altered.After Bifidobacterium treatment,there was a significant increase in Ac-tinobacteria and a significant decrease in Firmicutes of NAFLD patients.Bifidobacterium and Bacteroides were significantly in-creased,and Eubacterium_coprostanoligenes were significantly decreased.LEfSe analysis identified biomarkers of Bifidobacteri-aceae,Actinobacteria,Coriobacteriaceae,and Enterobacteriaceae after Bifidobacteriaceae treatment.Conclusion Bifidobacte-rium tetragenous viable bacteria tablets can regulate liver function and lipid metabolism and improve gut microbiota imbalance in NAFLD patients,which has a certain clinical application prospect.
NETL
NSTL
万方数据
