摘要
目的 了解中国旱獭、土拨鼠肝组织及外周血单核细胞(peripheral blood mononuclear cells,PBMC)中 Ⅰ型干扰素受体(type Ⅰ interferon receptor,mhIFNAR)的表达情况.方法 采用荧光定量 RT-PCR检测中国旱獭、土拨鼠肝组织、PBMC 中 mhIFNAR 的表达情况及其与感染状态是否存在相关性,以及 WH12-6、PWH细胞在 α、γ干扰素刺激下 mhIFNAR的表达情况.结果 中国旱獭肝组织、PBMC中 mhIFNAR表达,肝组织现症感染组及未感染组中 mhIFNAR1 和 mhIFNAR2 分别为 3.9±2.6、6.2±3.90(P=0.405),2.97±1.25、49.58±34.53(P=0.064),PBMC中分别为:5.675±3.076、5.18±1.16(P=0.694),32.04±13.01、4.35±1.44(P=0.000).α和 γ干扰素可以使 WH12-6 细胞中 mhIFNAR1 和 mhIFNAR2 分别上调(3.002±6.788)倍、(42.51±5.42)倍,而 γ干扰素分别上调(11.342±0.242)倍和(13.72±1.48)倍.土拨鼠不同感染状态下肝组织中 mhIFNAR 的表达如下:mhIFNAR1 和 mhIFNAR2 在慢性感染组、急性感染组、未感染组及合并 HDV感染组分别为 2.658±0.988、6.928±6.678、7.516±4.391、20.481±7.985(P=0.001);mhIFNAR2 则分别为 4.202±1.983、11.951±10.587、8.075±1.310、30.750±6.408(P=0.000);PWH 在 α、γ 干扰素刺激下,其中mhIFNAR2 分别上调(1.55±0.193)倍及(2.015±0.145)倍.结论 中国旱獭肝组织、PBMC可以检测到 mhIFNAR的表达,且 PBMC中 mhIFNAR2 的表达量与感染结局存在相关性,同样在土拨鼠肝组织内 mhIFNAR 的表达与感染状态存在相关性,慢性感染组偏低.而 α、γ干扰素可以上调 mhIFNAR的表达,为其抗病毒治疗的机制提供了新的依据.
Abstract
Objective To understand the expressions of mhIFNAR in liver tissues,peripheral blood mononuclear cells(PBMC)and the expression of mhIFNAR in WH12-6,PWH through the stimulation with α,γ interferon.Methods The expression of mhIFNAR in liver and PBMC were quantified by realtime RT-PCR.The WH12-6 and PWH cells were stimulated with α,γ interferon for 24 hours.And then the expression of mhIFNAR were also quantified by realtime RT-PCR.Results The mhIFNAR1 and mhIFNAR2 in liver tissues between current infection and uninfec-tion were 3.9±2.6,6.2±3.90(P=0.405);2.97±1.25,49.58±34.53(P=0.064)respectively,and in PBMC were 5.675±3.076,5.18±1.16(P=0.694);32.04±13.01,4.35±1.44(P=0.000).When the WH12-6 cells were stimulated α interferon,mhIFNAR1 and mhIFNAR2 were(3.002±6.788)times,(42.51±5.42)times higher than unstimulated cells,γ interferon also enhanced(11.342±0.242)times and(13.72±1.48)times,respectively.The mhIFNAR1 in woodchuck liver tissues among chronic carriers,actue infection,uninfecion and cofection HDV groups were 2.658±0.988,6.928±6.678,7.516±4.391 and 20.481±7.985(P=0.001),the mhIFNAR2 were 4.202±1.983,11.951±10.587,8.075±1.310 and 30.750±6.408(P=0.000).When the PWH were stimulated α and γ interferon,the mhIFNAR2 were(1.55±0.193)times and(2.015±0.145)times higher than unstimulated cells respec-tively.Conclusion The expression of mhIFNAR2 in PBMC is associated with the outcome of WHV infection in marmo-ta himalayana,and the expression of mhIFNAR in liver of woodchuck is low in WHV infection group.Last the α,γ in-terferon can upregulate the mhIFNAR in WH12-6 and PWH cells.This may explain the reason why different outcome of WHV infection,and explain the antiviral mechanism of α,γ interferon.
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