Study on expression of type Ⅰ interferon receptor in liver and peripheral blood mononuclear cells of marmota himalayana and woodchuck
Objective To understand the expressions of mhIFNAR in liver tissues,peripheral blood mononuclear cells(PBMC)and the expression of mhIFNAR in WH12-6,PWH through the stimulation with α,γ interferon.Methods The expression of mhIFNAR in liver and PBMC were quantified by realtime RT-PCR.The WH12-6 and PWH cells were stimulated with α,γ interferon for 24 hours.And then the expression of mhIFNAR were also quantified by realtime RT-PCR.Results The mhIFNAR1 and mhIFNAR2 in liver tissues between current infection and uninfec-tion were 3.9±2.6,6.2±3.90(P=0.405);2.97±1.25,49.58±34.53(P=0.064)respectively,and in PBMC were 5.675±3.076,5.18±1.16(P=0.694);32.04±13.01,4.35±1.44(P=0.000).When the WH12-6 cells were stimulated α interferon,mhIFNAR1 and mhIFNAR2 were(3.002±6.788)times,(42.51±5.42)times higher than unstimulated cells,γ interferon also enhanced(11.342±0.242)times and(13.72±1.48)times,respectively.The mhIFNAR1 in woodchuck liver tissues among chronic carriers,actue infection,uninfecion and cofection HDV groups were 2.658±0.988,6.928±6.678,7.516±4.391 and 20.481±7.985(P=0.001),the mhIFNAR2 were 4.202±1.983,11.951±10.587,8.075±1.310 and 30.750±6.408(P=0.000).When the PWH were stimulated α and γ interferon,the mhIFNAR2 were(1.55±0.193)times and(2.015±0.145)times higher than unstimulated cells respec-tively.Conclusion The expression of mhIFNAR2 in PBMC is associated with the outcome of WHV infection in marmo-ta himalayana,and the expression of mhIFNAR in liver of woodchuck is low in WHV infection group.Last the α,γ in-terferon can upregulate the mhIFNAR in WH12-6 and PWH cells.This may explain the reason why different outcome of WHV infection,and explain the antiviral mechanism of α,γ interferon.
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