Analysis of the diagnostic efficacy of m1A RNA methylation in rectal adenocarcinoma
Objective To explore the predictive value of m1A-related gene expression and overall plasma m1A methylation levels for the diagnosis and prognosis of rectal adenocarcinoma(READ).Methods Based on the Sangerbox(an online platform based on the TCGA and GTEx databases),the differential expression of 11 m1A methylation-related genes in READ was analyzed,and diagnostic efficacy and survival analysis were conducted in combination with clinical data.Meanwhile,the difference in plasma m1A methylation levels between READ patients and healthy individuals was detected to analyze its diagnostic value.Results TRMT6,a key m1A writer gene,was most significantly elevated in READ of different pathological states,and it can serve as a diagnostic marker,especially early stage READ,with a spe-cificity and sensitivity of up to 82.86％and 90.00％,respectively.Moreover,high expression of TRMT6 also indicated a better prognosis for READ(HR=0.22,95％CI:0.08-0.60,P=0.0014).Furthermore,plasma m1A methylation levels were significantly elevated in READ compared to healthy individuals,showing certain diagnostic value for early stage of READ,with AUC of about 0.8.Conclusion m1A-related genes and plasma m1A methylation are elevated in READ and can serve as potential early diagnostic markers.This study provided new targets for the early diagnosis of READ,which will help guide the development of more effective treatment strategies.
万方数据
维普
