Exploring the effects of exogenous hydrogen sulfide on hepatoma cell line HepG2 by RNA-seq
Objective To investigate the effects of exogenous hydrogen sulfide(H2S)on HCC and the possible mo-lecular mechanisms,and to provide experimental support for the diagnosis and treatment of HCC.Methods Gene ex-pression data of HCC were obtained from the GEO database.Transcriptomic data(RNA-seq)of HepG2 cells treated with exogenous H2S were analyzed to identify differentially expressed genes(DEGs).GO and KEGG pathway enrichment analyses were conducted to explore the main biological functions and pathways associated with these DEGs.Results GEO analysis revealed that the expression levels of key H2S-producing enzymes,cystathionine γ-lyase(CSE)and cystathi-onine β-synthase(CBS),were significantly reduced in HCC tissues compared to normal tissues.A total of 3 640 DEGs were identified,among which 317 were related to cellular senescence,580 to cell proliferation,and 43 to apoptosis.Protein-protein interaction network analysis highlighted Jun,MAPK1 and MAPK3 as key genes,suggesting that the effects of H2S on HCC may be associated with the Jun/MAPK signaling pathway.Conclusion H2S may regulate HCC development through the Jun/MAPK pathway,offering a basis for further exploration of H2S in the molecular mecha-nisms and potential therapeutic applications in HCC.
万方数据
维普
