The role and mechanism of taraxerol in protecting gastric epithelial cells by regula-ting AMPK pathway
Objective To investigate the protective effect of taraxerol on ethanol-injured gastric mucosal cells by AMPK signaling pathway by in vitro experimental study.Methods With ethanol to stimulate gastric mucosal cells as a model,taraxerol decoction to intervenet.The effect of taraxerol on the survival rate of gastric mucosa cells under the con-dition of ethanol stimulation was detected by CCK-8.The content of GSH-Px,SOD and MDA in gastric mucosa cells were measured by biochemical method.The cell cycle of gastric mucosa was detected by flow cytometry.Western blot-ting was used to detect the expression of AMPK,CaMKK and LKB1 protein.Results The activities of SOD and GSH-Px in gastric mucosal cells were significantly decreased and the content of MDA was significantly increased after the gas-tric mucosal cells were injured by ethanol.Cell proliferation was significantly decreased(P＜0.05),cells in G,phase decreased,and cells in S phase and G2 phase increased.The expression of AMPK,CaMKK and LKB1 in gastric muco-sal injury cells was also down-regulated.The activities of SOD and GSH-Px were significantly increased(P＜0.01),while the content of MDA was significantly decreased(P＜0.01).The cell proliferation was significantly enhanced(P＜0.05),the cells in G1 phase were increased,and the cells in S phase and G2 phase were decreased(P＜0.05).The expression levels of AMPK,CaMKK and LKB1 in gastric mucosal cells were up-regulated by ethanol in a concentration-dependent manner.Conclusion Taraxerol can effectively activate the AMPK pathway,thereby protecting the damaged gastric mucosa cells.
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