Effects of thyroid hormone T3 on proliferation and apoptosis of liver cells in mice with acute alcoholic liver injury
Objective To study the effects of thyroid hormone T3 on the liver structure and function,as well as the expression of genes related to cell proliferation and apoptosis in mice with acute alcoholic liver injury.Methods Nor-mal mice were intervened by intraperitoneal injection of different concentrations of thyroid hormone T3 in advance.After the last dose,50％alcohol solution was administered once by gavage.Twenty-four hours post-treatment,the mice were euthanized,and both serum and liver samples were collected.This experiment sought to establish a mouse model of acute alcoholic liver injury and assess the influence of thyroid hormone T3 on this condition.Results After T3 interven-tion,more severe damage in mice with acute alcoholic liver injury could be observed,clear liver lobule contours and neat arrangement of liver cords.But there was infiltration of inflammatory cells,increased serum ALT activity and liver ROS content,increased PCNA expression in the high-dose group(P＜0.05),and decreased expression of apoptotic genes Caspase3 and Bax compared to model group(P＜0.01).However,with the increase of T3 intervention concentra-tion,the expression of apoptotic genes showed an upward trend.Conclusion Intervention with high concentrations of thyroid hormone T3 could exacerbate acute alcoholic liver injury in mice.
Thyroid hormones T3Acute alcoholic liver injuryCell proliferation and apoptosis
万方数据
维普
