摘要
目的 分析川崎病患儿的肠道菌群分布特点.方法 选取 2022 年 3 月至12 月在潍坊市妇幼保健院儿科住院的11例川崎病患儿为观察组,选择同时期体检的 7 名健康儿童为对照组,使用16SrRNA测序分析粪便菌群,通过α、β多样性和各菌群的门、属水平相对丰度等分析川崎病儿童肠道菌群多样性和分布特点.结果 观察组肠道菌群丰富度高于对照组(P<0.05);观察组肠道菌群多样性高于对照组(P<0.05).通过Anosim分析明确两组肠道菌群具有统计学差异(P<0.05).在门水平,厚壁菌门在两组中均占优势,观察组中占比 38.9%,对照组中占比46.7%.在属水平,拟杆菌属、链球菌属、肠球菌属、乳酸杆菌属在观察组中的占比分别为 26.50%、4.29%、14.10%、0.14%,在对照组中为 3.73%、2.21%、30.60%、1.46%.在门水平,观察组拟杆菌门丰度增加(P<0.05).在属水平,拟杆菌属、粪杆菌属、布劳特氏菌属丰度增加(P<0.05);乳酸杆菌属、肠球菌属、纤毛菌属丰度减少(P<0.05).结论 川崎病患儿存在肠道菌群紊乱.
Abstract
Objective To analyze the characteristics of intestinal flora distribution in children with Kawasaki disease.Methods A total of 11 children with Kawasaki disease hospitalized in the Department of Pediatrics of Weifang Maternal and Child Health Hospital from March to December 2022 were selected as the observation group,and 7 healthy children who underwent physical examination during the same period were selected as the control group.The fecal flora was analyzed by 16SrRNA sequencing,and the intestinal flora diversity and distribution characteristics of children with Kawasaki disease were analyzed by α and β diversity,relative abundance of each flora at the gate and genus level,etc.Results The richness of intestinal flora in the observation group was higher than that in the control group(P<0.05).The diversity of intestinal flora in the observation group was higher than that in the control group(P<0.05).Anosim analysis showed that there were significant differences in intestinal flora between the two groups(P<0.05).At the gate level,Firmicutes were dominant in both groups,accounting for 38.9%in the observation group and 46.7%in the control group.At the genus level,Bacteroides,Streptococcus,Enterococcus and Lactobacillus accounted for 26.50%,4.29%,14.10%and 0.14%in the observation group,and 3.73%,2.21%,30.60%and 1.46%in the control group,respectively.At the gate level,the abundance of Bacteroidetes increased in the observation group(P<0.05).At the genus level,the abundances of Bacteroides,Faecalis and Brautella increased(P<0.05).The abundance of Lactobacillus,Enterococcus and Leptothrix decreased(P<0.05).Conclusion Children with Kawasaki disease have intestinal flora disorder.
基金项目
山东省潍坊市卫生健康委科研项目(WFWSJK-2023-06)
国家科技期刊平台
NSTL
万方数据