首页|Quantitative Analysis of Retinoic Acid Combining 2-Nitrophenylhydrazine Derivatization with LC-MS/MS

Quantitative Analysis of Retinoic Acid Combining 2-Nitrophenylhydrazine Derivatization with LC-MS/MS

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All-trans-retinoic acid (atRA) plays an essential role in organ formation and differentiation,tissue cell proliferation,and apoptosis,and is closely related to the occurrence and development of various diseases.Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has become a powerful tool for metabolic biological analysis because of the high sensitivity,specificity and throughput.We have developed a new method for the quantification of atRA by chemical derivatization with 2-nitrophe-nylhydrazine (2-NPH) for the first time,which reduced the interference of detection by changing the ion mass of the molecule.atRA from its endogenous geometric isomers,such as,9-cis-RA and 13-cis-RA,has been baseline resolved within 15 minute under the optimized chromatographic conditions.The method was applied to measure atRA in mouse serum and tissues including liver,kidney,brain,pancreas,and subcutaneous tissue.The method is easy to operate,highly sensitive and well selective,and can be used for accurate and rapid determination of atRA in biological samples.

all-trans-retinoic acid2-nitrophenylhydrazinederivatizationliquid chromatography-tandem mass spectrometry (LC-MS/MS)

DENG Yinghua、YOO Hongsik、ZHANG Hongquan、YANG Di、NAPOLI Joseph

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Department of Chemistry and Life Science, Hubei University of Education, Wuhan 430205, Hubei, China

Hubei Engineering Technology Research Center of Environmental Purification Materials, Wuhan 430205, Hubei, China

Department of Nutritional Sciences and Toxicology,University of California, Berkeley, California 94720, USA

Supported by the College Outstanding Young Scientific and Technological Innovation Team of Hubei Province

T201718

2020

10.19823/j.cnki.1007-1202.2020.0027

武汉大学自然科学学报(英文版)
武汉大学

武汉大学自然科学学报(英文版)

CSTPCDCSCD
影响因子：0.066
ISSN：1007-1202
年,卷(期)：2020.25(3)
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