首页|Meta-Analysis of Polymerase Chain Reaction in the Detection of Human Leptospirosis

Meta-Analysis of Polymerase Chain Reaction in the Detection of Human Leptospirosis

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
  • 维普
Leptospirosis,a major zoonotic disease widespread in the world,would infect humans and other animals by direct contacting with the soil or water contaminated by the pathogenic leptospires.This report analyzes the accuracy of polymerase chain reaction (PCR) assay used in the detection of leptospira specific genes in humans.Seventeen published articles which included 2 526 samples satisfied all inclusion standards and were applied to meta-analysis.Pooled sensitivity and specificity were 0.73 (95％ CI 0.70-0.76) and 0.94 (95％ CI 0.93-0.95),respectively.Heterogeneity was statistically significant among studies,but none of the sources for heterogeneity (disease stage,PCR type,targeted genes) could adequately interpret this finding.Meta-regression advised that real-time PCR (qPCR) targeted 16s rRNA would be the best choice for early detection of cases during the acute stage of the disease and was accounted as a good screening tool in all stages of the disease.

human leptospirosispolymerase chain reactionmeta-analysissensitivityspecificity

MA Lina、MA Ya、LUO Bailing、MA Bishu、YAN Jie、LIN Xu'ai

展开 >

School of Medicine, Zhejiang University, Hangzhou 310058, Zhejiang, China

Department of Medical Teaching in Dingxi Campus, Gansu University of Chinese Medicine, Dingxi 743000, Gansu,China

Department of Petroleum Chemical Engineering, Lanzhou Petrochemical College of Vocational Technology, Lanzhou 730060,Gansu, China

Department of Medical Microbiology and Immunology,Haiyuan School of Kunming Medical University, Kunming 650106, Yunnan, China

展开 >

National Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Provinceand the Higher Education Institutions of Gansu Province of China

81271781LY17H1900022019B-230

2020

10.19823/j.cnki.1007-1202.2020.0054

武汉大学自然科学学报(英文版)
武汉大学

武汉大学自然科学学报(英文版)

CSTPCDCSCD
影响因子：0.066
ISSN：1007-1202
年,卷(期)：2020.25(5)
  • 37