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三峡库区冬季河岸带土壤微生物群落结构及功能

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人为周期性调控水库运行条件下,河岸带生境复杂,其土壤微生物特性研究甚少.本研究选取三峡库区干流河岸带土壤为研究对象,于水库调度运行高水位时期,探索土壤微生物沿程变化特征及其影响因素,并预测它们在土壤生态系统中的关键功能.研究发现,微生物群落的α多样性指数随着河岸带与大坝的距离增大而逐渐增加,在上下游不同的处理间,细菌β多样性有着显著性差异(P＜0.001),真菌无显著性差异(P＞0.05);群落组成相似性随Bray-Curtis距离的增加而逐渐降低(细菌,P＜0.001;真菌,P＞0.05);PICRUSt2功能预测分析表明,土壤细菌主要功能以代谢(Metabolism,78.64％)相关通路为主,碳代谢相关的酶功能基因的表达远高于氮代谢酶功能基因的表达;硝酸还原酶基因在氮代谢中发挥了重要作用,Armati-monadota 门和髌骨菌门(Patescibacteria)以及螺旋体门(Spirochaetota)可作为冬季河岸带土壤微生物代谢途径的标记性物种.
Structure and Function of Winter Soil Microbial Communities in Riparian Zone of the Three Gorges Reservoir Area
The riparian habitat undergoes complex changes due to the artificial periodic operation of reservoirs,and little is known about the characteristics of riparian soil microorganisms during the winter season.In this study,riparian zones were selected as the research object during the high water level period of reservoir operation to investigate the variation characteristics in soil microorganisms,explore the driving factors,and predict key functions along the Three Gorges Reservoir(TGR).The results revealed that the a-diversity index of microbial communities increased with the distance from the dam.Between the upstream and downstream,the β-diversity of bacteria showed a significant difference(P＜0.001)while fungi exhibited no significant difference(P＞0.05).The similarity of community composition decreased as the Bray-Curtis distance increased(Bacteria:P＜0.001;Fungi:P＞0.05).PICRUSt2 function prediction showed that the main function of soil bacteria was related to metabolism pathways(78.64％),and the expression of carbon metabolism-related enzyme functional genes was much higher than that of nitrogen metabolism-related enzyme functional genes.Additionally,the nitrate reductase gene played a crucial role in nitrogen metabolism,and Armatimonadota,Patescibacteria and Spirochaetota could be used as marker taxa of the microbial metabolic pathway in winter riparian zones.

the Three Gorges Reservoir Areariparian zone soilmicrobial communitiessignificantly differential speciesfunctional genesmetabolism

杨一宁、陈垚、张媛媛、李哲、鲁伦慧

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中国科学院重庆绿色智能技术研究院,重庆 400714

重庆交通大学河海学院,重庆 400074

三峡库区 河岸带土壤 微生物群落 显著性差异物种 功能基因 代谢

国家自然科学基金青年基金水利部重大计划项目中国科学院"西部之光"人才培养计划"西部青年学者"项目

42107273SKS-2022081

2024

10.14188/j.1671-8836.2023.0062

武汉大学学报(理学版)
武汉大学

武汉大学学报(理学版)

CSTPCD北大核心
影响因子：0.814
ISSN：1671-8836
年,卷(期)：2024.70(1)
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