茵陈原儿茶酸乙酯对非酒精性脂肪肝的作用及机制
Effect and Mechanism of Ethyl 3,4-Dihydroxybenzoate from Artemisia capillaris Thunb.Aerial Part on Non-Alcoholic Steatohepatitis
沈婉莹 1魏晓芳 1姚杰 2刘晨霄 2张春 3陆梦柯 3唐旭东 3葛岚岚4
作者信息
- 1. 深圳市人民医院龙华分院中心实验室,广东深圳 518120;甘肃中医药大学药学院,甘肃兰州 730000;深圳清华大学研究院创新中药及天然药物研究重点实验室,广东深圳 518000
- 2. 深圳市人民医院龙华分院中心实验室,广东深圳 518120
- 3. 甘肃中医药大学药学院,甘肃兰州 730000;深圳清华大学研究院创新中药及天然药物研究重点实验室,广东深圳 518000
- 4. 深圳市人民医院龙华分院中心实验室,广东深圳 518120;深圳市人民医院龙华分院病理科,广东深圳 518120
- 折叠
摘要
茵陈是一种清利湿热,利胆退黄的药食同源中药.本研究通过H2O2氧化应激细胞损伤模型从茵陈中筛选出一种具有显著抗氧化活性的成分原儿茶酸乙酯(EDHB),再通过棕榈酸/油酸(PA/OA)诱导的体外细胞模型模拟脂肪肝中脂滴形成,进一步验证了 EDHB的体外抗非酒精性脂肪性肝炎(NASH)作用.体内动物实验通过蛋氨酸-胆碱缺乏(MCD)饮食喂养构建的NASH大鼠模型来评价.结果显示,茵陈中EDHB能有效改善NASH大鼠肝组织病理变化,减轻脂肪变性,降低肝脏转氨酶水平,抑制肝脏炎症因子的表达及转录,并通过激活肝脏核因子红细胞系2相关因子2(Nrf2),有效缓解NASH.
Abstract
Artemisia capillaris Thunb.aerial part is a kind of food homologous traditional Chinese medicine which can clear away dampness and heat,promote gallbladder and reduce jaundice.In this study,ethyl 3,4-dihydroxybenzoate(EDHB),a component with significant antioxidant activity,was screened from A.capillaris aerial part by H2O2 oxidative stress cell injury model,and then the formation of lipid droplets in fatty liver was simulated by palmitic acid/oleic acid(PA/OA)further verified the anti-non-alcoholic steatohepatitis(anti-NASH)effect of EDHB in vitro.Animal experiment,established by NASH rat model induced by Methionine choline deficiency(MCD)diet,was used to evaluate the effect of EDHB in vivo.Results showed that EDHB from Artemisia capillaris aerial part could effectively alleviate NASH by improving the pathological changes,reducing the steatosis,lowering the transaminase levels,as well as inhibiting the expression and transcription of inflammatory factors including nuclear factor erythroid 2-related factor 2(Nrf2)in liver tissue.
关键词
茵陈/原儿茶酸乙酯/氧化应激/炎症/Nrf2/非酒精性脂肪肝Key words
Artemisia capillaris Thunb.aerial parts/ethyl 3,4-dihydroxybenzoate/oxidative stress/inflammation/Nrf2/non-alcoholic steatohepatitis引用本文复制引用
基金项目
国家自然科学基金(81903760)
深圳市基础研究面上项目(JCYJ20190806151816859)
出版年
2024
NETL
NSTL
万方数据