泥鳅皮冻干粉对CC14诱导小鼠肝损伤的保护作用
Protective Effect of Lyophilized Powder of Loach Skin on CCI4-Induced Liver Injury in Mice
欧阳敬禹 1袁毅 1郑佳贝 1尤祥宇 1苏江涛 1糜志远 1肖红卫 2吴艳2
作者信息
- 1. 湖北工业大学生物工程与食品学院,湖北武汉 430068
- 2. 湖北省农业科学院畜牧兽医研究所/动物胚胎工程及分子育种湖北省重点实验室,湖北武汉 430064
- 折叠
摘要
通过腹腔注射CC14诱导建立小鼠肝损伤模型,研究了口服泥鳅皮冻干粉(LPLS)对肝损伤小鼠血清中肝功能标志物、肝组织中氧化应激标志物水平、肝组织的病理学、肝细胞凋亡水平、肝组织中抗氧化应激和细胞凋亡相关信号通路主要参与者mRNA与蛋白表达变化的影响.研究发现,口服LPLS能以剂量依赖的方式,显著改善CC14引起的小鼠肝组织病理性变化和肝细胞凋亡程度,显著降低肝损伤小鼠血清中天冬氨酸转氨酶(AST)和丙氨酸转氨酶(ALT)的活性,降低肝组织中丙二醛(MDA)含量,增加超氧化物歧化酶(SOD)活性和还原型谷胱甘肽(GSH)含量;在mRNA和蛋白水平分别上调其核红细胞2相关因子2(Nrf2)、血红素加氧酶1(HO-1)、B淋巴细胞瘤-2(Bcl-2),下调其kelch样ECH相关蛋白1(Keap-1)和Bcl-2相关X蛋白(Bax)的表达.因此,LPLS可通过调节氧化应激、肝细胞凋亡发挥肝脏保护作用.
Abstract
Mouse liver injury model was induced by intraperitoneal injection of CCl4.The effects of oral administration of lyophi-lized powder of loach skin(LPLS)on the liver function biomarkers in serum,oxidative stress biomarkers in tissue,histopathology changes,the hepatocyte apoptosis level,and the mRNA and protein expression of main factors involving in anti-oxidative stress or apoptosis pathways in liver tissue were studied.This study revealed that LPLS intake significantly improved the histopathologic modification and hepatocyte apoptosis caused by CC14 in a dose-dependent manner,also significantly reduced the levels of alanine transaminase(ALT)and aspartate transaminase(AST),decreased the level of malondialdehyde(MDA),as well as increased the activity of superoxide dismutase(SOD)and the content of glutathione(GSH)in liver.Furthermore,LPLS upregulated the expres-sion of nuclear factor erythroid 2-related factor 2(Nrf2),heme oxygenase-1(HO-1),B-cell lymphoma-2(Bcl-2)and downregu-lated the expression of kelch-like ECH-associated protein 1(Keap-1)and Bcl-2-associated X(Bax)on both mRNA and protein level.In short,LPLS can protect the liver by regulating both of oxidative stress and hepatocyte apoptosis in vivo.
关键词
泥鳅/冻干粉/肝损伤/氧化应激/凋亡Key words
Misgurnus anguillicaudatus/lyophilized powder/liver injury/oxidative stress/apoptosis引用本文复制引用
基金项目
国家自然科学基金(81703409)
动物胚胎工程及分子育种湖北省重点实验室开放基金(KLAEMB-2019-02)
出版年
2024
NETL
NSTL
万方数据