Protective Effect of Lyophilized Powder of Loach Skin on CCI4-Induced Liver Injury in Mice
Mouse liver injury model was induced by intraperitoneal injection of CCl4.The effects of oral administration of lyophi-lized powder of loach skin(LPLS)on the liver function biomarkers in serum,oxidative stress biomarkers in tissue,histopathology changes,the hepatocyte apoptosis level,and the mRNA and protein expression of main factors involving in anti-oxidative stress or apoptosis pathways in liver tissue were studied.This study revealed that LPLS intake significantly improved the histopathologic modification and hepatocyte apoptosis caused by CC14 in a dose-dependent manner,also significantly reduced the levels of alanine transaminase(ALT)and aspartate transaminase(AST),decreased the level of malondialdehyde(MDA),as well as increased the activity of superoxide dismutase(SOD)and the content of glutathione(GSH)in liver.Furthermore,LPLS upregulated the expres-sion of nuclear factor erythroid 2-related factor 2(Nrf2),heme oxygenase-1(HO-1),B-cell lymphoma-2(Bcl-2)and downregu-lated the expression of kelch-like ECH-associated protein 1(Keap-1)and Bcl-2-associated X(Bax)on both mRNA and protein level.In short,LPLS can protect the liver by regulating both of oxidative stress and hepatocyte apoptosis in vivo.
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