Radicals Scavenging and Enzymes Inhibitory Activities of Total Flavonoid from Leaves of Scutellaria amoena
The total flavonoid from leaves of Scutellaria amoena(SALF)was obtained and the content was determined.The radicals scavenging,α-glucosidase and pancreatic lipase inhibitory activities of SALF were investigated.The results showed that the content of total flavonoid was 492.1±15.5 mg/g by spectrophotometry with rutin as a reference.The 50％scavenging concen-trations(SC50)of SALF on DPPH radicals(DPPH·),ABTS radicals(ABTS·+),hydroxyl radical(OH·)and nitric oxide radical(NO·)were 57.58±7.10,20.71±1.63,44.40±2.74,and 104.61±9.39 μg/mL,respectively.The 50％inhibitory concentrations of enzymes activities(IC50)of SALF on α-glucosidase from Saccharomyces cerevisiae(α-glucosidase-Y),α-glucosidase from rats small intestine(α-glucosidase-R),and pancreatic lipase were 228.6±4.61,128.4±5.83,and 22.41±1.13 μg/mL,respectively.The results after statistical analyzed indicate that SALF has a certain degree of radicals scavenging,α-glucosidase and pancreatic lipase inhibitory activities.
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