滇黄芩叶总黄酮的自由基清除及抑酶活性
Radicals Scavenging and Enzymes Inhibitory Activities of Total Flavonoid from Leaves of Scutellaria amoena
姚新成 1王皎 2骆瑞玲 3胡英2
作者信息
- 1. 云南中医药大学基础医学院,云南昆明 650500
- 2. 云南中医药大学中药学院,云南昆明 650500
- 3. 云南中医药大学人文与管理学院,云南昆明 650500
- 折叠
摘要
从滇黄芩(Scutellaria amoena)叶中提取黄酮类成分,经制备获得滇黄芩叶总黄酮(SALF),检测其含量,并考察了SALF清除自由基及抑制α-葡萄糖苷酶、胰脂肪酶活性的能力.结果显示,SALF中黄酮含量为492.1±15.5mg/g.SALF清除DPPH自由基(DPPH·)、ABTS自由基(ABTS·+)、羟基自由基(OH·)和一氧化氮自由基(NO·)的半清除率(SC50)分别为57.58±7.10、20.71±1.63、44.40±2.74、104.61±9.39 μg/mL.对酿酒酵母α-葡萄糖苷酶(α-glucosidase-Y)活性的半抑制浓度(IC50)为228.6±4.61 µg/mL,对大鼠肠道α-葡萄糖苷酶(α-glucosidase-R)活性的半抑制浓度为128.4±5.83 µg/mL;对胰脂肪酶活性的半抑制浓度为22.41士 1.13 µg/mL.统计分析表明,SALF具有一定的自由基清除和α-葡萄糖苷酶、胰脂肪酶活性的抑制能力.
Abstract
The total flavonoid from leaves of Scutellaria amoena(SALF)was obtained and the content was determined.The radicals scavenging,α-glucosidase and pancreatic lipase inhibitory activities of SALF were investigated.The results showed that the content of total flavonoid was 492.1±15.5 mg/g by spectrophotometry with rutin as a reference.The 50％scavenging concen-trations(SC50)of SALF on DPPH radicals(DPPH·),ABTS radicals(ABTS·+),hydroxyl radical(OH·)and nitric oxide radical(NO·)were 57.58±7.10,20.71±1.63,44.40±2.74,and 104.61±9.39 μg/mL,respectively.The 50％inhibitory concentrations of enzymes activities(IC50)of SALF on α-glucosidase from Saccharomyces cerevisiae(α-glucosidase-Y),α-glucosidase from rats small intestine(α-glucosidase-R),and pancreatic lipase were 228.6±4.61,128.4±5.83,and 22.41±1.13 μg/mL,respectively.The results after statistical analyzed indicate that SALF has a certain degree of radicals scavenging,α-glucosidase and pancreatic lipase inhibitory activities.
关键词
滇黄芩/总黄酮/自由基清除/α-葡萄糖苷酶/胰脂肪酶Key words
Scutellaria amoena/total flavonoid/radicals scavenging/α-glucosidase/pancreatic lipase引用本文复制引用
出版年
2024
NETL
NSTL
万方数据