不同产地金银花初生代谢物化学成分的GC-MS分析
Chemical Composition Analysis of Primary Metabolites of Lonicera japonica Flos from Different Habitats by GC-MS
程喜真 1张玉玲 1郭浩川 1郑玉光 2郭慧 1马东来3
作者信息
- 1. 河北中医药大学药学院,河北石家庄 050200
- 2. 河北中医药大学药学院,河北石家庄 050200;河北省中药炮制技术创新中心,河北石家庄 050200
- 3. 河北中医药大学药学院,河北石家庄 050200;河北省中药炮制技术创新中心,河北石家庄 050200;河北省中药资源利用与质量评价国际联合研究中心,河北石家庄 050091
- 折叠
摘要
利用柱前衍生化气相色谱-质谱联用技术(GC-MS)结合主成分分析(PCA)以及正交偏最小二乘法判别分析(OPLS-DA)等方法对不同产地金银花初生代谢物的化学成分进行了比较,并进行代谢通路的富集分析,探讨了不同产地金银花初生代谢物化学成分的差异代谢机制.共确定了 28种初生代谢物、10种初生差异代谢物以及3条主要差异代谢通路.结果表明,不同产地金银花的初生代谢物存在明显差异;不同产地金银花的初生差异代谢物在氨酰-tRNA生物合成,缬氨酸、亮氨酸和异亮氨酸生物合成以及甘氨酸、丝氨酸和苏氨酸代谢途径中存在着显著差异.该方法可为快速区分金银花产地提供理论依据.
Abstract
The chemical composition of primary metabolites of Lonicera japonica flos from various producing regions was compared using gas chromatography-mass spectrometry(GC-MS)along with principal component analysis(PCA)and orthogonal partial least square discriminant analysis(OPLS-DA).Additionally,metabolic pathway enrichment analysis was conducted to study the differential metabolic mechanisms of primary metabolites of Lonicera japonica flos from different habitats.A total of 28 primary metabolites,including 10 primary differential metabolites and 3 major differential metabolic pathways,were identified.The findings revealed significant variations in the primary metabolites of Lonicera japonica flos from distinct producing areas,particularly in aminoacyl-tRNA biosynthesis,valine,leucine,and isoleucine biosynthesis,as well as glycine,serine,and threonine metabolic pathways.This approach can serve as a basis for efficiently distinguishing the geographical origin of Lonicera japonica flos.
关键词
金银花/代谢组学/初生代谢物/柱前衍生化/气相色谱-质谱联用技术(GC-MS)Key words
Lonicera japonica flos/metabolomics/primary metabolites/pre-column derivatization/gas chromatography-mass spectrometry(GC-MS)引用本文复制引用
基金项目
河北省重点研发计划(19276414D)
河北省中医药局项目(2019075)
河北省现代农业产业技术体系建设项目中药材创新团队项目(HBCT2018060205)
河北中医学院大学生创新创业训练计划(202014432036)
出版年
2024
NETL
NSTL
万方数据