Molecular Cloning and Expression of ScTIP1;1 in Stipa capillacea under Abiotic Stress
Based on EST sequence and RACE experiments,the full-length coding sequence of a tonoplast intrinsic protein (TIP) gene termed ScTIP1; 1 was cloned from Stipa capillacea.Sequence analysis showed that the opening reading frame (ORF) of ScTIP1; 1 was 753 bp,encoding a 250 amino acid peptide with a protein molecular weight of 25.8 kD and isoelectric point of 6.16.Phylogenetic analysis and sequence alignment results indicated that ScTIP1 ;1 had a close relationship with AtTIP1 ;1 of Arabidopsis thaliana.Subcellular localization analysis indicated that ScTIP1 ;1 was localized in the tonoplast membrane.The expression level of ScTIP1;1 was induced by salt,drought and especially low temperature in the leaves of S.capillacea.These results provide new insight into understanding the ecological adaptations of S.capillacea.
NETL
NSTL
万方数据
维普
