Identification and expression analysis of VQ family genes in Lolium perenne L.
Valine-glutamine(VQ)is a plant-specific protein,which plays an important role in plant growth,de-velopment,and resistance to biotic and abiotic stresses.To date,however,little research has been conduc-ted on the VQ gene family in perennial ryegrass(Lolium perenne L.).In this study,52 LpVQ genes were iden-tified in the perennial ryegrass genome,which were unevenly distributed on seven chromosomes.Subcellular localization prediction indicated that LpVQ proteins were mainly located in the nucleus,with only LpVQ45 lo-cated in the cell membrane.Phylogenetic analysis showed that LpVQ genes were divided into seven subfami-lies and there were only four pairs of lineal homologous genes with Arabidopsis.Based on gene structure analysis of the LpVQ gene family,88.46%of LpVQ genes had no intron.Finally,three LpVQ genes were ran-domly from each subfamily to observe the responses to dark,high temperature,and salt stress.The qRT-PCR analysis LpVQ genes responded quickly in early stages of stress.This study provides a theoretical foundation for further study on VQ family genes of perennial ryegrass under abiotic stress.
NETL
NSTL
万方数据
维普
