Gardenia jasminoides J.Ellis flowers are white in color and have a pleasant aroma.These flowers have high ornamental value and are an important source of natural spices.Terpenes are the main compo-nents of the unique aroma of G.jasminoides flowers,but the key terpene synthases(TPSs)for the biosynthe-sis of such volatile products have not yet been identified.Here,based on the high-quality genome of G.jasmi-noides,this study comprehensively explored the TPS genes related to floral scent biosynthesis through tran-scriptomic,phylogenetic,and conserved domain analysis.In total,44 GjTPS genes were identified in G.jasmi-noides,unevenly distributed on 11 chromosomes,and nine GjTPS genes participated in tandem duplication events.All GjTPSs were clustered into five subfamilies,27 of which belonged to the angiosperm-specific clades,including TPS-a,TPS-b,and TPS-g.Combining transcriptomic data and quantitative real-time PCR(qRT-PCR)analyses of five organs,five candidate GjTPS genes,with high expression in blooming flowers and containing the active motif DDXXD and NSE/DTE,were screened.Among them,GjTPS1,GjTPS2,GjTPS3,and GjTPS27 were grouped in the TPS-b clade,speculated to be the TPSs responsible for the biosynthesis of the main floral aroma components of G.jasminoides,such as linalool and ocimene,while GjTPS18 from the TPS-a clade may be a farnesene synthase.
NETL
NSTL
维普
万方数据
