摘要
以前期在铜污染矿区修复中筛选出的耐铜樟树(Cinnamomum camphora(L.)Presl)为材料,通过盆栽模拟实验,以不添加铜为对照(CK),设置50、150、300、600、900、1 200 mg/kg 6个处理浓度,胁迫处理60 d后,测定樟树的生物量积累、叶绿素含量、生理生化指标及樟树对铜离子的富集和转移量,分析不同浓度铜处理对耐铜樟树生长、生理生化的影响,以及铜在不同组织的富集转运规律.结果显示,樟树对铜胁迫表现出低浓度(150 mg/kg)促进生长,高浓度抑制生长的趋势.超氧化物歧化酶(Superoxide dismutase,SOD)、过氧化物酶(Peroxidase,POD)、过氧化氢酶(Catalase,CAT)、过氧化氢(Hydrogen peroxide,H2O2)、游离脯氨酸和可溶性糖含量等生理指标在 900 mg/kg处理时没有显著升高;谷胱甘肽还原酶(Glu-tathione reductase,GR)随着处理浓度的升高逐渐降低;丙二醛(Malondialdehyde,MDA)、谷胱甘肽(Glutathione,GSH)含量则随着处理浓度的升高逐渐升高.各器官铜含量的大小顺序为根>叶>茎,樟树主要将铜富集在根系,往地上茎和叶的转移率很低,极大地降低了对茎、叶的铜毒害作用,且随着处理浓度的增加,铜由根系向叶片的转移率IF(Leaf/Root)由对照处理的 0.078逐降至 1 200 mg/kg处理时的 0.007;铜由根系向茎的转移率IF(Stem/Root)由对照处理的0.06逐降至1 200 mg/kg处理时的0.005.综合其生长、生理及铜的富集转运特性,樟树对铜具有较好的耐受性,耐受浓度可达900 mg/kg,是可用于铜污染土壤种植的理想材料.
Abstract
This study investigated copper-resistant Cinnamomum camphora(L.)Presl in the restoration of a copper-polluted mining area through pot simulation experiments.Six treatment concentrations were estab-lished(50,150,300,600,900,and 1 200 mg/kg),with no copper addition used as the control(CK).After 60 d of treatment,biomass accumulation,chlorophyll content,physiological and biochemical indexes of C.campho-ra,and copper ion accumulation and transfer were measured.The impact of different copper concentrations on growth,physiological,and biochemical responses in C.camphora,as well as the patterns of copper accu-mulation and distribution in various tissues,were explored to evaluate copper tolerance in this species.Re-sults showed that C.camphora growth under copper stress was enhanced at low copper concentrations(150 mg/kg)and inhibited at high copper concentrations.Physiological indicators,including superoxide dismu-tase(SOD),peroxidase(POD),catalase(CAT),hydrogen peroxide(H2O2),free proline,and soluble sugar,did not significantly increase under 900 mg/kg copper treatment.glutathione reductase(GR)decreased gradually with the increase of copper concentration,while malondialdehyde(MDA)and glutathione(GSH)contents in-creased gradually with the increase of copper concentration.Copper was predominantly accumulated in the roots,minimizing toxicity to above-ground tissues,with organ copper content in the order root>leaf>stem.The copper transfer rate(IF)from root to leaf(leaf/root)decreased from 0.078 in the control to 0.007 in the 1 200 mg/kg copper treatment group,while the copper transfer rate(IF)from root to stem(Stem/Root)de-creased from 0.06 in the control to 0.005 in the 1 200 mg/kg copper treatment group.Based on growth perfor-mance,physiological responses,and copper ion accumulation and transfer,C.camphora exhibited good cop-per tolerance,withstanding up to 900 mg/kg,highlighting its potential for planting in copper-contaminated soils.
基金项目
江西省重点研发计划(20202BBFL63049)
江西省科学院重点科研项目(2018-YZD2-05)
江西省科学院省级科研院基础研究项目(2023YJC2004)
维普
万方数据