Growth and physiological response of Cinnamomum camphora(L.)Presl to copper stress and analysis of copper enrichment and transport characteristics
This study investigated copper-resistant Cinnamomum camphora(L.)Presl in the restoration of a copper-polluted mining area through pot simulation experiments.Six treatment concentrations were estab-lished(50,150,300,600,900,and 1 200 mg/kg),with no copper addition used as the control(CK).After 60 d of treatment,biomass accumulation,chlorophyll content,physiological and biochemical indexes of C.campho-ra,and copper ion accumulation and transfer were measured.The impact of different copper concentrations on growth,physiological,and biochemical responses in C.camphora,as well as the patterns of copper accu-mulation and distribution in various tissues,were explored to evaluate copper tolerance in this species.Re-sults showed that C.camphora growth under copper stress was enhanced at low copper concentrations(150 mg/kg)and inhibited at high copper concentrations.Physiological indicators,including superoxide dismu-tase(SOD),peroxidase(POD),catalase(CAT),hydrogen peroxide(H2O2),free proline,and soluble sugar,did not significantly increase under 900 mg/kg copper treatment.glutathione reductase(GR)decreased gradually with the increase of copper concentration,while malondialdehyde(MDA)and glutathione(GSH)contents in-creased gradually with the increase of copper concentration.Copper was predominantly accumulated in the roots,minimizing toxicity to above-ground tissues,with organ copper content in the order root>leaf>stem.The copper transfer rate(IF)from root to leaf(leaf/root)decreased from 0.078 in the control to 0.007 in the 1 200 mg/kg copper treatment group,while the copper transfer rate(IF)from root to stem(Stem/Root)de-creased from 0.06 in the control to 0.005 in the 1 200 mg/kg copper treatment group.Based on growth perfor-mance,physiological responses,and copper ion accumulation and transfer,C.camphora exhibited good cop-per tolerance,withstanding up to 900 mg/kg,highlighting its potential for planting in copper-contaminated soils.
Cinnamomum camphoraCopper stressPhysiological responseEnrichment and transport
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