首页|药用植物遗传转化和基因编辑技术研究进展

药用植物遗传转化和基因编辑技术研究进展

扫码查看
植物遗传转化和基因编辑技术是后基因组时代研究基因功能的重要手段.植物遗传转化从最初依赖组织培养的方式发展到了不依赖组织培养的直接转化方式;基因编辑技术从需要剪切特定基因片段再修复的第1阶段编辑技术,发展到了无需剪切即可在特定部位精确替换碱基或精确插入、删除片段以及大片段的第3阶段编辑技术,两种技术不断取得突破,推动着植物生物技术的飞速发展.本文综述了植物遗传转化方法与基因编辑技术的最新研究进展,以及它们在药用植物中的应用,并对药用植物遗传转化新方法和基因编辑技术的建立进行了展望,旨在为药用植物的基因功能和分子育种研究提供技术支撑.
Recent progress in medicinal plant transformation and genome editing
In the post-genomic era,both plant genetic transformation and genome editing have emerged as critical tools for gene function research.Plant genetic transformation has evolved from methods requiring tis-sue culture to new approaches that bypass tissue culture entirely.Furthermore,gene editing technology has advanced from initial techniques of targeted gene segment modification and repair to more sophisticated strategies,such as precise nucleotide substitution and targeted insertion,deletion,and segment editing at specific sites without the need for cutting.Recent advancements in both plant genetic transformation and genome editing have significantly accelerated the field of plant biotechnology.In the present paper,we review recent progress on plant genetic transformation and genome editing technologies,as well as their applica-tions in medicinal plants,providing insights into gene function analysis and molecular breeding strategies for medicinal plants.

Medicinal plantsGenetic transformationGene editing

张云罗、吴迎梅、刘义飞、胡志刚、苟君波

展开 >

湖北中医药大学药学院,湖北省中药资源与中药化学重点实验室,湖北省时珍实验室,武汉 430065

云南师范大学生命科学学院,马铃薯科学研究院,云南省马铃薯生物学重点实验室,昆明 650500

湖北江夏实验室,武汉 430200

药用植物 遗传转化 基因编辑

深圳市科创委基础研究项目湖北省自然科学基金中医药创新发展联合基金重点项目湖北中医药大学重大科技攻关计划

JCYJ201908131416016612023AFD1212023ZDXM007

2024

植物科学学报
中国科学院武汉植物园, 湖北省植物学会

植物科学学报

CSTPCD北大核心
影响因子:1.934
ISSN:2095-0837
年,卷(期):2024.42(2)
  • 16