毛茛中与苯丙烷生物合成途径相关的1R-MYB类转录因子的鉴定与分析
Identification and analysis of 1R-MYB transcription factors regulating the phenylpropane biosynthesis pathway in Ranunculus japonicus Thunb.
樊金世博 1钟欣欣 1单婷玉 1张京晶 1王郅炜 1吴家文2
作者信息
- 1. 安徽中医药大学研究生院,合肥 230012;安徽中医药大学科研实验中心,新安医学教育部重点实验室,合肥 230038
- 2. 安徽中医药大学科研实验中心,新安医学教育部重点实验室,合肥 230038;安徽省中医药科学院,合肥 230012;安徽道地中药材品质提升协同创新中心,合肥 230012
- 折叠
摘要
1R-MYB家族在植物生长发育、抗胁迫、次生代谢物合成中具有重要的调控作用.本研究基于毛茛(Ranunculus japonicus Thunb.)的全长转录组测序数据库,分析了其1R-MYB家族成员的基序、系统进化关系、表达特性、空间结构及功能.结果共鉴定出221个1R-MYB基因,其中18个通过KEGG富集到苯丙烷生物合成途径,可能调控此途径中的过氧化物酶.研究结果为深入探究毛茛1R-MYB转录因子调控苯丙烷的生物合成机制奠定了基础.
Abstract
The 1R-MYB family plays a crucial role in plant growth,development,stress resistance,and se-condary metabolite synthesis.A full-length transcriptome database for three different tissues of Ranunculus japonicus Thunb.was constructed using PacBio isoform sequencing and DNA nanoball sequencing.In this study,members of the 1R-MYB family were identified in the full-length transcriptome database of R.japonicus.Their motifs,family evolution,expression patterns,spatial structure,and functions were analyzed.In total,221 MYB-related genes were identified at the transcriptome level,with 18 1R-MYB transcription factors(TFs)found to be enriched in the phenylpropanoid biosynthesis pathway based on KEGG analysis,potentially regu-lating peroxidase activity.Overall,this study provides a foundation for further exploration of the regulatory functions of 1R-MYB TFs in secondary metabolite biosynthesis in R.japonicus.
关键词
毛茛/转录因子/全长转录组/1R-MYB/苯丙烷生物合成途径Key words
Ranunculus japonicus/Transcription factors/Full-length transcriptome/1R-MYB/Phenyl-propanoid biosynthesis引用本文复制引用
基金项目
国家重点研发计划项目(2017YFC1701600)
安徽省自然科学基金项目(2008085MH268)
安徽省高等学校科学研究项目(2022AH050474)
出版年
2024
NETL
NSTL
万方数据