锰掺杂纳米羟基磷灰石的体外抗菌-促成骨性能研究
In vitro Antibacterial and Osteogenic Properties of Manganese Doped Nano Hydroxyapatite
李承瑜 1丁自友 1韩颖超1
作者信息
- 1. 武汉理工大学 材料复合新技术国家重点实验室,武汉 430070
- 折叠
摘要
羟基磷灰石(HAP)作为一种常见的骨修复材料,在治疗感染性骨缺损时仍面临着细菌感染的风险,有限的骨诱导能力也阻碍了其进一步应用.本研究采用共沉淀法制备了一种锰掺杂羟基磷灰石纳米棒(MnHAP),该纳米材料兼具优良的细胞生物相容性,高抗菌效率和骨诱导能力.抗菌实验表明,MnHAP10(n(Mn)/n(Ca+Mn)=10%))对大肠杆菌和金黄色葡萄球菌的抑制率可分别达到77.85%和75.92%,并且在808 nm近红外光照射下,对大肠杆菌的抗菌效率得到了进一步增强(97.63%).细胞增殖以及相关成骨基因实验表明,MnHAP有利于成骨细胞的增殖分化,提高了对蛋白质的吸附能力和成骨活性,促进了相关成骨基因的表达,且具有良好的生物相容性.MnHAP 纳米棒有望为感染性骨缺损治疗提供一种新的思路.
Abstract
Hydroxyapatite(HAP),as a common bone repair material,still faces the risk of bacterial infection in the treatment of infectious bone defects,whose limited osteogenic properties also hinders its further application.This study used a coprecipitation method to prepare the manganese doped hydroxyapatite nanorod(MnHAP),which exhibited excellent cell biocompatibility,high antibacterial efficiency and osteogenic properties.Antibacterial experiments showed that the inhibition rates of MnHAP10(n(Mn)/n(Ca+Mn)=10%))against Escherichia coli and Staphylococcus aureus can reach 77.85%and 75.92%,respectively.Moreover,the antibacterial efficiency of MnHAP10 against Escherichia coli can be further enhanced(97.63%)under 808 nm near-infrared light irradiation.Cell proliferation and related osteogenic gene experiments display that MnHAP is beneficial for the proliferation and differentiation of osteoblasts,which improves protein adsorption capacity,stimulates osteogenic activity,and promotes the expression of related osteogenic genes,demonstrating its good biocompatibility.Therefore,MnHAP nanorods are expected to provide a new approach in the treatment of infectious bone defects.
关键词
羟基磷灰石/锰/抗菌/感染性骨缺损/光热Key words
hydroxyapatite/manganese/antibacterial/infectious bone defect/photothermal引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据