小粒径Fe3O4-DMSA-PEI磁性纳米颗粒的制备及其基因负载能力研究
Fe3O4-DMSA-PEI Magnetic Nanoparticles with Small Particle Size:Preparation and Gene Loading
蔡和庆 1韩璐 1杨松松 1薛新玉 1张扣 1孙志成 1刘儒平 1胡堃 1危岩2
作者信息
- 1. 北京印刷学院印刷与包装工程学院,北京 102600
- 2. 清华大学生命有机磷化学及化学生物学教育部重点实验室,北京 100084
- 折叠
摘要
四氧化三铁(Fe3O4)磁性纳米颗粒因其制备简单,在外加磁场作用下具有靶向性,并且表面易接枝等特性,可作为被动靶向载体应用于基因治疗领域.本研究采用溶剂热法制备纳米颗粒,并调控堆积生长时间,制得粒径在4～9 nm范围内可控的油相Fe3O4纳米颗粒;使用内消旋-2,3-二巯基丁二酸(DMSA)二次取代其表面的油酸分子,使其具备良好的水相分散性;通过酰胺化反应在其表面接枝支链型聚乙烯亚胺(PEI),最终得到Fe3O4-DMSA-PEI磁性纳米颗粒.研究发现,Fe3O4-DMSA-PEI磁性纳米颗粒的表面Zeta电位高达(52.50±1.94)mV,具有一定的超顺磁性(14.48 emu/g,1 emu/g=1 A·m2/kg).磁性纳米颗粒与质粒DNA的质量比为15∶1时可完全阻滞DNA在凝胶上的电泳,装载量高达6.67％.本研究制备的Fe3O4-DMSA-PEI磁性纳米颗粒具有一定的基因负载能力,有望作为基因载体应用于基因转染领域.
Abstract
Ferroferric oxide(Fe3O4)magnetic nanoparticles are widely used as passive targeting carriers in gene therapy,due to their simple preparation,targeting under external magnetic field and easy surface grafting.This study synthesized oil phase Fe3O4 nanoparticles with controllable particle sizes in the range from 4 to 9 nm by regulating the accumulation growth time in the solvothermal method.Then,meso-2,3-dimercaptosuccinic(DMSA)was employed to double exchange oleic acid molecules on its surface to provide good water dispersibility.Finally,Fe3O4-DMSA-PEI magnetic nanoparticles were obtained by grafting branched polyethylenimine(PEI)onto Fe3O4-DMSA surface through amidization reaction.The results demonstrate that the Fe3O4-DMSA-PEI magnetic nanoparticles have a surface Zeta potential of(52.50±1.94)mV,remaining a certain degree of superparamagnetism(14.48 emu/g,1 emu/g=1 A·m2/kg).When the mass ratio of Fe3O4-DMSA-PEI magnetic nanoparticles to plasmid DNA is 15∶1,it can completely block DNA and its loading capacity is as high as 6.67％.The Fe3O4-DMSA-PEI magnetic nanoparticles prepared in this study have a certain gene delivery ability and are expected to be used as gene carriers in the field of gene transfection.
关键词
四氧化三铁/磁性纳米颗粒/基因载体/表面改性Key words
ferroferric oxide/magnetic nanoparticle/gene carrier/surface modification引用本文复制引用
基金项目
国家自然科学基金(61971049)
国家自然科学基金(62211530446)
国家自然科学基金(22278037)
北京印刷学院科研创新团队项目(Ea202205)
北京市高教学会立项重点项目(2023)(ZD202312)
出版年
2024
国家科技期刊平台
NSTL
万方数据