具有红色荧光性质的掺硒碳点在生物传感和抗菌中的多功能应用
Red-emitting selenium-doped carbon dots for versatile applications in biosensing and antibacterial activities
厉圆圆 1卢修联 1刘欣雨 1张磊 1景苏1
作者信息
- 1. 南京工业大学化学与分子工程学院,南京 211816
- 折叠
摘要
以易得的苯亚硒酸(BA)和邻苯二胺(o-PDA)作为前体,通过一步水热法成功设计并合成了掺杂硒的碳点(Se-CDs).制备的Se-CDs发出红色波段的荧光,并被用于开发一种检测microRNA-21(miR-21)的灵敏荧光传感器.通过脱氧核糖核酸酶Ⅰ(DNase Ⅰ)介导的信号放大方法,将miR-21的检测限从78 nmol·L-1改进到了6.8 nmol·L-1.此外,Se-CDs对大肠杆菌等革兰氏阴性菌具有理想的抑制活性.
Abstract
In this study,we successfully designed and synthesized selenium-doped carbon dots(Se-CDs)using easi-ly available precursors of benzeneseleninic acid(BA)and o-phenylenediamine(o-PDA)through a one-step hydro-thermal method.These Se-CDs emitted red-wavelength fluorescence and were utilized to develop a sensitive fluores-cence sensor for detecting microRNA-21(miR-21).By incorporating deoxyribonuclease Ⅰ(DNase Ⅰ)-mediated signal amplification,the detection limit for miR-21 was improved from 78 to 6.8 nmol·L-1.Furthermore,we observed desirable inhibitory activity of the Se-CDs against Gram-negative bacteria of Escherichia coli(E.coli).
关键词
掺硒碳点/红色荧光/生物传感/microRNA-21/抗菌Key words
selenium-doped carbon dots/red fluorescence/biosensing/microRNA-21/antibacterial引用本文复制引用
基金项目
国家自然科学基金(22175090)
国家自然科学基金(22374075)
江苏省重点研发计划(BE2021712)
出版年
2024
NETL
NSTL
维普
万方数据