A Benzoxazole-based turn-on fluorescent probe for visual detection of N2H4
A turn-on fluorescent probe(HL2)for visual detection of N2H4 was synthesized and characterized using benzoxazole as a fluorophore,and its spectral properties were investigated.The experimental results indicated that HL2 had high selectivity and sensitivity to N2H4,strong anti-interference ability,and a wide pH response range(5-10).HL2 quickly recognized N2H4 within 2 min,accompanied by a color change of the probe solution from colorless to blue.The fluorescent intensity of HL2 showed excellent linear relationship with the concentration of N2H4(0.1-25μmol·L-1),and its detection limit was evaluated to be 0.072 μmol·L-1.In addition,HL2 can be applied to detecting N2H4 in real water samples,and it could also realize fluorescence imaging of N2H4 in HeLa cells.CCDC:2268187.
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