无机化学学报2024,Vol.40Issue(2) :353-360.DOI:10.11862/CJIC.2023.189

一种可视化检测肼的苯并噁唑类荧光增强型探针

A Benzoxazole-based turn-on fluorescent probe for visual detection of N2H4

王汉林 郑汉 黄杰勋 黄卫东 杨龙元 李武
无机化学学报2024,Vol.40Issue(2) :353-360.DOI:10.11862/CJIC.2023.189

一种可视化检测肼的苯并噁唑类荧光增强型探针

A Benzoxazole-based turn-on fluorescent probe for visual detection of N2H4

王汉林 1郑汉 1黄杰勋 1黄卫东 2杨龙元 1李武1
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作者信息

  • 1. 湖北理工学院环境科学与工程学院,黄石 435003;矿区环境污染控制与修复湖北省重点实验室,黄石 435003
  • 2. 湖北理工学院化学与化工学院,黄石 435003
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摘要

以苯并噁唑为荧光基团,合成和表征了一种用于可视化检测N2H4的荧光增强型探针(HL2),并研究了其光谱性质.实验结果表明,HL2对N2H4具有高的选择性和灵敏度,抗干扰能力强,响应快,它对N2H4的检测限为0.072 μmol·L-1.此外,HL2可'应用于实际水体样品中N2H4的检测,并且还能实现HeLa细胞中N2H4的荧光成像.

Abstract

A turn-on fluorescent probe(HL2)for visual detection of N2H4 was synthesized and characterized using benzoxazole as a fluorophore,and its spectral properties were investigated.The experimental results indicated that HL2 had high selectivity and sensitivity to N2H4,strong anti-interference ability,and a wide pH response range(5-10).HL2 quickly recognized N2H4 within 2 min,accompanied by a color change of the probe solution from colorless to blue.The fluorescent intensity of HL2 showed excellent linear relationship with the concentration of N2H4(0.1-25μmol·L-1),and its detection limit was evaluated to be 0.072 μmol·L-1.In addition,HL2 can be applied to detecting N2H4 in real water samples,and it could also realize fluorescence imaging of N2H4 in HeLa cells.CCDC:2268187.

关键词

苯并噁唑/荧光探针//可视化检测/荧光成像

Key words

benzoxazole/fluorescent probe/hydrazine/visual detection/fluorescent imaging

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基金项目

国家自然科学基金(42107088)

湖北省大学生创新创业训练项目(S202210920015)

中央引导地方科技发展资金项目(2022BGE252)

湖北省教育厅科学技术研究计划青年人才项目(Q20214505)

矿区环境污染控制与修复湖北省重点实验室开放基金(2013101)

出版年

2024
无机化学学报
中国化学会

无机化学学报

CSTPCDCSCD北大核心
影响因子:0.665
ISSN:1001-4861
参考文献量21
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