基于双靶向黑色素纳米粒的肿瘤深穿透光声成像

Deeply penetrating photoacoustic imaging in tumor tissues based on dual-targeted melanin nanoparticle

曲波涛 ¹王倩 ²宁晓刚 ³周雨欣 ¹张瑞平⁴

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作者信息

1. 山西医科大学基础医学院,太原 030001
2. 山西医科大学法医学院,太原 030001
3. 山西中医药大学中药与食品工程学院,晋中 030619
4. 山西医科大学第一医院放疗科,太原 030001
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摘要

通过1-(3-二甲基氨基丙基)-3-乙基碳二亚胺和N-羟基丁二酰亚胺反应将透明质酸(hyaluronic acid,HA)和4-羧丁基三苯基溴化膦((4-carboxybutyl)triphenylphosphonium bromide,TPP)分子连接到聚乙二醇-氨基(PEG-NH2)修饰过的黑色素纳米颗粒(melanin nanoparticles,MNP)表面,设计合成了一种具有双靶向能力的黑色素纳米颗粒(MNP-TPP-HA),赋予了黑色素的双靶向能力.体外三维(3D)多细胞肿瘤球荧光成像和活体肿瘤光声成像实验表明MNP-TPP-HA具有优异的肿瘤靶向穿透能力.

Abstract

A dual-targeting melanin nanoparticle(MNP-TPP-HA)was constructed by grafting the carboxyl groups of hyaluronic acid(HA)and(4-carboxybutyl)triphenylphosphonium bromide(TPP)onto the surface of polyethylene glycol-amino(PEG-NH2)modified MNP based on 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride(EDC)and N-hydroxy succinimide(NHS)reaction,endowing melanin with the ability of dual active targeting.The fluorescence imaging of in vitro three-dimensional(3D)tumor models and in vivo photoacoustic imaging(PAI)all reveal the excellent target penetration ability of MNP-TPP-HA.

关键词

双靶向/黑色素/光声成像/穿透能力

Key words

dual-targeting/melanin/photoacoustic imaging/penetration ability

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基金项目

国家自然科学基金(82120108016)

国家自然科学基金(82071987)

国家万人计划项目(SQ2022RA2A300118)

Research Project Supported by Shanxi Scholarship Council of China(2020-177)

Fund Program for the Scientific Activities of Selected Returned Overseas Professionals in Shanxi Province(20200006)

Four Batches of Scientific Research Projects of Shanxi Provincial Health Commission(2020TD11)

Four Batches of Scientific Research Projects of Shanxi Provincial Health Commission(2020SYS15)

Four Batches of Scientific Research Projects of Shanxi Provincial Health Commission(2020XM10)

Key Laboratory of Nanoimaging and Drugloaded Preparation of Shanxi Province(202104010910010)

出版年

2024

无机化学学报

中国化学会

无机化学学报

CSTPCD北大核心

影响因子：0.665

ISSN：1001-4861

参考文献量32

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