The improved Hummers method was used to obtain GOQDs from flake graphite,which contained rich hydroxyl and carboxyl groups.With the as-fabricated GOQDs in hand,we constructed a GOQDs/OVA nano-vaccine using chicken ovalbumin(OVA)as a model antigen to evaluate the immune efficacy and safety.Results showed that GOQDs/OVA nano-vaccine had high water dispersibility and stability with a diameter of around 5 nm for 30 d.The maximum loading capacity of GOQDs for OVA was about 500 mg·g-1,and release rates of OVA were 74.65％and 56.93％in pH 5.5 and 7.4 after 24 h,respectively,displaying pH stimulus responsive release merits.With the con-centration of GOQDs below 500 μg·mL-1,the biosecurity of GOQDs indicated that they were not causing hemolysis,cell damage,and pathological changes in important tissues.After immunization,GOQDs/OVA nano-vaccines could excite the high levels of immunoglobulin G(IgG),immunoglobulin G1(IgG 1),and immunoglobulin G2a(IgG2a)anti-bodies and improve secretions of interleukin-1 β(IL-1β),interleukin-4(IL-2),interleukin-4(IL-4),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),and interferon-γ(IFN-γ),compared with the group of OVA alone.Mean-while,GOQDs promoted an increase in the percentage of CD4+and CD8+T lymphocytes in the spleen.
维普
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