Inhibitory effect of Desmodium styracifolium polysaccharides with different content of carboxyl groups on the growth,aggregation and cell adhesion of calcium oxalate crystals
To explore the regulatory effect of Desmodium styracifolium polysaccharides before and after carboxy-methylation(DSPs)on the nucleation,growth and aggregation of calcium oxalate(CaC2O4)crystals,the CaC2O4 crys-tals regulated by DSPs with different carboxyl(—COOH)contents(mass fractions)were characterized by FTIR,pow-der X-ray diffraction,scanning electron microscope,ζ potential and thermogravimetric analysis(TGA).The protec-tive effect of DSPs on human renal proximal tubular epithelial cells(HK-2 cells)was evaluated by examining adhe-sion molecule expression and nano-COM(nano calcium oxalate monohydrate)cell adhesion.The results show that DSPs can inhibit the growth of COM crystals,induce the formation of calcium oxalate dihydrate(COD)crystals,increase the absolute value of ζ potential on the surface of CaC2O4 crystals,and inhibit aggregation between crystals.When the—COOH content in the polysaccharide increased from 1.17％to 7.45％,12.2％and 17.7％,the ability of DSP0,DSP1,DSP2 and DSP3 to regulate the growth of CaC2O4 crystals increased in sequence.TGA shows that poly-saccharides are adsorbed in the crystal.In the presence of 0.2 g·L-1 DSPs,the mass fractions of DSP0,DSP1,DSP2 and DSP3 incorporated into the crystals were 1.54％,2.94％,7.96％and 8.12％,respectively.Cell experiments show that DSPs can significantly reduce the expression of adhesion molecules CD44 and Annexin A2 on the surface of HK-2 cells,and reduce the amount of nano-COM adhesion on the cell surface.DSPs can inhibit the nucleation,aggregation,and growth of CaC2O4 crystals,and reduce the adhesion of nano-COM on renal epithelial cells,all these are beneficial to inhibit the formation of CaC2O4 kidney stones,among which DSP3 with the highest degree of car-boxylation has the best effect.
calcium oxalatecrystallization regulationcrystal adhesion moleculescanning electron microscopekidney stone
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