pH/光热双重响应的纳米递送系统构建及对耐药膀胱癌细胞的联合治疗

Construction of pH/photothermal dual-responsive delivery nanosystem for combination therapy of drug-resistant bladder cancer cell

吴頔 ¹史芮萌 ²王朝阳 ¹师跃华 ¹杨帆 ³曾乐勇¹

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作者信息

1. 河北大学化学与材料科学学院,河北省化学生物学重点实验室,药物化学与分子诊断教育部重点实验室,保定 071000
2. 河北大学基础医学院,保定 071000
3. 河北大学附属医院泌尿外科,保定 071000
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摘要

以介孔聚多巴胺(MPDA)的制备为出发点,通过搭载化疗药物阿霉素(DOX)和包覆相变材料1-十四醇(PCM),构建了pH/光热双重响应的MPDA-DOX@PCM纳米递送系统,实现了对耐药膀胱癌细胞(BIU-87/ADR)的光热治疗(PTT)和化疗.结果表明,MPDA-DOX@PCM尺寸约为179 nm,DOX的最大搭载率为22％,光热转换效率高达49.1％.在pH=7.4和温度为25 ℃的条件下,DOX的累积释放率为4.57％;当pH值降为5.5和温度升高到45℃时,DOX的累积释放率可提高到60.13％.在808 nm激光辐照下,MPDA-DOX@PCM孵育的BIU-87/ADR细胞存活率降低至9.5％,证明了其优异的PTT/化疗联合治疗性能.

Abstract

Mesoporous nanomaterials showed potential application in drug delivery,but non-specific leakage and multi-drug resistance seriously restricted the efficacy of chemotherapy.Based on the synthesis of mesoporous poly-dopamine(MPDA),pH/photothermal dual-responsive MPDA-DOX@PCM delivery nanosystem was constructed by loading chemotherapy drug doxorubicin(DOX)and coating phase-change material(PCM)1-tetradecanol,by which the photothermal therapy(PTT)and chemotherapy were achieved towards drug-resistant bladder cancer cell(BIU-87/ADR).The results indicated that the size of MPDA-DOX@PCM was about 179 nm,the max loading rate of DOX was 22％,and the photothermal conversion efficiency was up to 49.1％.Under the conditions of pH=7.4 and 25 ℃,the accumulative release rate of DOX was 4.57％,but can increase to 60.13％with the conditions of pH=5.5 and 45 ℃.Under the irradiation of 808 nm laser,the viability of BIU-87/ADR cells incubated with MPDA-DOX@PCM decreased to 9.5％,demonstrating the excellent combination performance of PTT/chemotherapy.

关键词

介孔聚多巴胺/药物递送/双重响应/联合治疗/膀胱癌细胞

Key words

mesoporous polydopamine/drug delivery/dual-response/combination therapy/bladder cancer cell

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基金项目

国家自然科学基金(U20A20254)

河北省自然科学基金(2024201004)

中央引导地方科技发展资金项目(226Z1302G)

出版年

2024

无机化学学报

中国化学会

无机化学学报

CSTPCD北大核心

影响因子：0.665

ISSN：1001-4861

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