The outbreaks of waterbome cryptosporidiosis is worldwidely happened continuously in recent years. The oocysts, the resistant stage of Cryptosporidium, are remarkably stable. The infective dose is low. The infection is always more serious in immunosuppression persons, and there is no special effect drug in clinical. Detecting the oocysts' quantity, species and viability become the main task in the control of infections caused by this pathogen. The internationally recommended method for the detection of Cryptosporidium is the EPA's 1622 or 1623 method. But this method's cost is high, process is trival and it can not judge the oocysts1 species and viability. Various molecular biological detection methods such as different kinds of PCR methods, LAMP method, nucleic acid hybridization technology and others have became the important means and beneficial supplement of the traditional methods because of their general characteristics of high speed, high sensitivity and specificity, as well as low cost in the Cryptosporidium detection in water samples. Especially they generally have the advantages in the judgement of oocysts' species and identification their viability, therefore can be also used in the Cryptosporidium infection sources' tracking and risk assessment of the environmental contamination. Among them, the LAMP technology because of its rapid reaction, good specificity, high sensitivity and low cost advantages, is the most promising.
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