Single Cell Capture Culture and Precise Printing Based on Microfluidic Technology
Single cells are basic units of complex biological systems.Due to the heterogeneity among cells,the population analysis often fails to reflect the behavior of single cell.A microfluidic chip was proposed,which could isolate single-cell from cell suspensions and had a large enough space to allow long-term stable cell culture.After the cells have grown for a period of time,the complementary metal oxide semiconductor(CMOS)circuit drives the underlying chip to heat the corresponding resistance,which prints the cells onto the substrate for biological analysis.When the cell suspension concentration is 5 × 106 cells/mL and the cell sedimentation time is 3 min,the capture efficiency of single cell reaches 38％,and the captured cell can be stably cultured in the chip for more than 72 h and can be precise printed.The new microfluidic chip is easy to operate and low cost,which provides a new idea for single cell analysis.
single cellmicrofluidic chipcell capturestable cultureprecise printing
万方数据
维普
