Effects of BDNF on improving cognitive dysfunction in rats with sepsis-associated encephalopathy
Objective:To investigate the effects of brain derived neurotrophic factor(BDNF) on the learning and memory function in rats with sepsis and the potential mechanisms.Methods:Forty male SD rats with sepsis induced by cecal ligation and puncture (CLP) were randomized into sham operation group (sham group),SAE model group(CLP group),BDNF group(B group) and TrkB receptor antagonist group(N group).Before model establishment,rats in the sham group and CLP group were injected with saline via intracerebroventricle in dose of 5μL,and those in B group were administered with BDNF(2 ng/μL) in dose of 5μL.Rats in N group were treated with the same volume of TrkB receptor antagonist (NTrk2,1 μg/μL).Morris water maze test was performed to test the place navigation of rats from day 2 to 6,and space exploration test was performed by day7.Results:Compared to the sham group,rats in CLP and N groups had longer escape latency(P<0.05),and those in CLP,B and N groups had significantly reduced percentage of crossing target quadrant and exploring frequencies(P<0.05).Rats in CLP and N groups had reduced frequencies of platform crossing compared to those in sham group(P<0.05),whereas rats in B group had higher number of times of crossing platform than those in CLP group(P<0.05).The swimming velocity remained no statistical difference in four groups.Conclusion:Damaged cognitive function of rats with sepsis-associated encephalopathy can be reversed by BDNF.The potential mechanism may be involved in BDNF-TrkB signaling pathway.
sepsis-associated encephalopathybrain derived neurotrophic factorMorris water maze
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NSTL
万方数据
维普
