YTHDC1 mediated CENPK regulating human cervical cancer cell invasion and proliferation
Objective:To identify the prediction and treatment targets of cervical cancer,and investigate the relationship between these targets and N6-methyladenosine(m6A)nucleic acid modification and their respective biological functions.Methods:GEPIA2 database was used to screen differentially expressed genes in cervical cancer.EdU assay,scratch assay and Transwell assay were used to detect the effects of CENPK on the proliferation,migration,and invasion of cervical cancer cells.The relationship between m6A modification and high expression of CENPK was studied by combining quantitative determination of RNA m6A modification with Western blot.Results:CENPK expression was upregulated in cervical cancer tissues(P＜0.05).Silencing the CENPK gene resulted in decreased proliferation,migration and invasion of cervical cancer cells(P＜0.01).The m6A reader protein YTHDC1 was involved in cervical cancer by reducing the expression of CENPK protein(P＜0.01).Conclusion:These results suggest that CENPK is a potential therapeutic target in cervical cancer.
cervical cancercentromere protein KYTH domain-containing protein 1tumor cellinvasionproliferation
万方数据
维普
