首页|C5aR在金黄色葡萄球菌LukS-PV诱导非小细胞肺癌细胞凋亡中的作用

C5aR在金黄色葡萄球菌LukS-PV诱导非小细胞肺癌细胞凋亡中的作用

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目的:探讨金黄色葡萄球菌杀白细胞素S组分(LukS-PV)通过活化补体5受体(C5aR)对非小细胞肺癌(NSCLC)细胞A549和H460凋亡的影响.方法:将敲低C5aR表达的慢病毒载体转染至A549和H460细胞中,qRT-PCR和Western blot检测C5aR敲低率,Annexin V/PI染色法检测敲低C5aR对细胞凋亡的影响.对敲低C5aR的细胞再加入LukS-PV,通过Annexin V/PI染色法和凋亡相关蛋白检测细胞凋亡情况.结果:与PBS对照组相比,LukS-PV可促进A549和H460细胞凋亡(P<0.05).慢病毒载体转染A549和H460细胞敲低C5aR后,细胞C5aR表达水平降低(P<0.05).在敲低C5aR基础上再加入LukS-PV,细胞凋亡受到抑制(P<0.05).结论:敲低C5aR可抑制LukS-PV诱导NSCLC细胞的凋亡.
Role of C5aR in the apoptosis of non-small cell lung cancer cells induced by LukS-PV
Objective:To investigate the effect of staphylococcal bicomponent LukS-PV leukocidin on the apoptosis of non-small cell lung cancer(NSCLC)cell lines A549 and H460 mediated by the C5a receptor(C5aR).Methods:Lentiviral vector with knockdown C5aR was transfected into A549 and H460 cells.qRT-PCR and Western blot were performed to measure the knockdown rate of C5aR.Annexin V/PI staining was used to determine the effect of C5aR knockdown on the cell apoptosis.Cells with C5aR knockdown were treated with LukS-PV.Annexin V/PI staining and apoptosis related proteins were then used to detect the cell apoptosis.Results:Compared with PBS control group,LukS-PV significantly promoted the apoptosis of A549 and H460 cells(P<0.05).C5aR expression was markedly decreased after knockdown of C5aR in the A549 and H460 cells transfected with lentiviral vector(P<0.05),and the apoptosis was inhibited when the cells further treated with LukS-PV after knockdown of C5aR(P<0.05).Conclusion:Knockdown of C5aR can inhibit LukS-PV induced apoptosis in NSCLC cells.

C5 a receptorLukS-PVnon-small cell lung cancercell apoptosis

张鹏、王杨燕、李婕

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皖南医学院第一附属医院弋矶山医院 检验科,安徽 芜湖 241001

活化补体5受体 金黄色葡萄球菌杀白细胞素S组分 非小细胞肺癌 细胞凋亡

皖南医学院重点项目科研基金

WK2021ZF20

2024

皖南医学院学报
皖南医学院

皖南医学院学报

CSTPCD
影响因子:0.695
ISSN:1002-0217
年,卷(期):2024.43(1)
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