Objective:To investigate the effect of staphylococcal bicomponent LukS-PV leukocidin on the apoptosis of non-small cell lung cancer(NSCLC)cell lines A549 and H460 mediated by the C5a receptor(C5aR).Methods:Lentiviral vector with knockdown C5aR was transfected into A549 and H460 cells.qRT-PCR and Western blot were performed to measure the knockdown rate of C5aR.Annexin V/PI staining was used to determine the effect of C5aR knockdown on the cell apoptosis.Cells with C5aR knockdown were treated with LukS-PV.Annexin V/PI staining and apoptosis related proteins were then used to detect the cell apoptosis.Results:Compared with PBS control group,LukS-PV significantly promoted the apoptosis of A549 and H460 cells(P＜0.05).C5aR expression was markedly decreased after knockdown of C5aR in the A549 and H460 cells transfected with lentiviral vector(P＜0.05),and the apoptosis was inhibited when the cells further treated with LukS-PV after knockdown of C5aR(P＜0.05).Conclusion:Knockdown of C5aR can inhibit LukS-PV induced apoptosis in NSCLC cells.
关键词
活化补体5受体/金黄色葡萄球菌杀白细胞素S组分/非小细胞肺癌/细胞凋亡
Key words
C5 a receptor/LukS-PV/non-small cell lung cancer/cell apoptosis
维普
万方数据