Objective:To investigate the efficacy and safety of laparoscopic liver resection(LLR)without portal occlusion.Methods:Respective analysis was performed in 100 cases of liver tumor undergone LLR in our hospital between 2016 and 2021.The patients were divided into portal occlusion group(treated with portal occlusion),and non-portal occlusion group(without portal occlusion).The data,including operative time,intraoperative blood loss and perioperative biochemical indicators,were recorded and analyzed in the two groups.Results:LLR was successfully completed in the 100 patients without conversion to open surgery.Patients in the portal occlusion group had lower intraoperative blood loss and higher postoperative transaminase level than those in non-portal occlusion group(P<0.05).Bile leakage occurred in 3 cases after operation,and was managed by peritoneal lavage and drainage.No perioperative death or severe complications occurred,and there was no incidence of unplanned reoperation.All patients were safely discharged,and subjected to follow-up for 6 to 12 months,which showed no recurrence and distant metastasis.Conclusion:Laparoscopic liver resection without portal occlusion can be safe and feasible in eligible patients,and reduce the effect of hepatic portal occlusion on postoperative liver function.