Asiatic acid inhibits migration of lung adenocarcinoma cells through Wnt/β-catenin signaling pathway
Objective:To study the effect of asiatic acid on the migration of lung adenocarcinoma cell lines H1299 and A549 and its related molecular mechanism.Methods:H1 299 and A549 cells were treated with different doses of asiatic acid(0,20,40,60,80,100μmol/L),and cell survival rate was measured by MTT assay.Apoptosis was detected by flow cytometry.Cell scratch test was performed to observe the effect of asiatic acid on cell migration.Western blot was used to detect the effects of asiatic acid treatment on Wnt/β-catenin signaling pathway and EMT-related protein expression in cells.Results:MTT assay showed that the survival rates of H1299 and A549 cells were decreased with the increase of asiatic acid concentration and treatment time(P<0.05).Flow cytometry showed that asiatic acid could promote apoptosis(P<0.05).The results of cell scratch test showed that asiatic acid inhibited the migration of H1299 and A549 cells(P<0.05).Western blot results indicated that the expression of β-catenin,p-GSK-3βSer9,c-myc,Vimentin and Snail was down-regulated with added drug dose,whereas the expression of Axin2 and E-cadherin was increased.Conclusion:Asiatic acid can check the tumor cell migration by inhibiting epithelial-mesenchymal transition(EMT)in lung adenocarcinoma cells.The mechanism may be related to the inhibition of Wnt/β-catenin signaling pathway.