Investigation on the molecular mechanism of honokiol in promoting apoptosis of lung cancer cells
Objective:To investigate the effect of honokiol(HNK)on the apoptosis of H1299 and H460 cells,and preliminarily explore its related molecular mechanisms.Methods:Non-small cell lung cancer(NSCLC)H1299 and H460 cells were cultured in vitro and treated with different concentration of HNK for 48 hours.MTT and flow cytometry were used to respectively detect the cell viability,apoptosis and cycle inhibition.Western blot was performed to measure the protein expression levels of TAZ,PARP,Bcl-2,and caspase-3.Results:After treatment with HNK,the viability of H1299 and H460 cells was decreased in all concentration groups and at all treatment time point compared to the control group(both P＜0.05).Western blot determination revealed that the expression levels of TAZ and Bcl-2 proteins were down-regulated with added HNK concentration,yet the expression levels of cleaved-caspase3 and cleaved-PARP proteins were increased with increment of HNK concentration,and the expression levels of caspase-3 and PARP proteins did not show any significant changes with addition of HNK concentration.After treatment with different concentration of HNK,the apoptosis rate amplified(P＜0.05),and overexpression of TAZ resulted in decrease in cell apoptosis rate compared to the negative control group(P＜0.05).Conclusion:HNK can promote apoptosis of NSCLC H1299 and H460 cells,and its mechanism may be mediated by inhibition of TAZ.
honokiolcell apoptosistranscriptional coactivator with PDZ-binding motifnon-small cell lung cancer
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