Short-chain chlorinated paraffins inducing neurotoxicity in PC 12 cells through the ROS/NF-κB pathway
Objective:To investigate the neurotoxic mechanism of short-chain chlorinated paraffins(SCCPs).Methods:PC12 cells were initially exposed to SCCPs at different concentrations(0,50,100,200,300 μg/L,respectively)for 24 hours,and then observed under a microscope and assayed using CCK-8 for their growth density and viability.Reactive oxygen species(ROS)and malondialdehyde(MDA)were used to detect cellular oxidative damage.Western blot was conducted to detect the protein expression of iNOS,COX-2,p65 and p-p65.qRT-PCR was used to detect the transcription levels of inflammatory cytokines IL-1 β,IL-6,and TNF-α.Results:Microscopy and CCK-8 analysis results showed that the density and viability of PC 12 cells were decreased upon exposing to 200 μg/L SCCPs(P＜0.01),and the relative levels of intracellular ROS and MDA were increased under the same exposure conditions(P＜0.001).Under NAC intervention,the expression of NF-κB pathway-related proteins and mRNAs was inhibited(P＜0.05).Conclusion:SCCPs can induce neurotoxicity in PC12 cell via ROS/NF-κB pathway.
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