首页|Regulation of RNase E during the UV stress response in the cyanobacterium Synechocystis sp.PCC 6803

Regulation of RNase E during the UV stress response in the cyanobacterium Synechocystis sp.PCC 6803

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Endoribonucleases govern the maturation and degradation of RNA and are indispensable in the posttranscriptional regulation of gene expression.A key endoribonuclease in Gram-negative bacteria is RNase E.To ensure an appropriate supply of RNase E,some bacteria,such as Escherichia coli,feedback-regulate RNase E expression via the rne 5'-untranslated region(5'UTR)in cis.However,the mechanisms involved in the control of RNase E in other bacteria largely remain unknown.Cyanobacteria rely on solar light as an energy source for photosynthesis,despite the inherent ultraviolet(UV)irradiation.In this study,we first investigated globally the changes in gene expression in the cyanobacterium Synechocystis sp.PCC 6803 after a brief exposure to UV.Among the 407 responding genes 2h after UV exposure was a prominent upregulation of rne mRNA level.Moreover,the enzymatic activity of RNase E rapidly increased as well,although the protein stability decreased.This unique response was underpinned by the increased accumulation of full-length rne mRNA caused by the stabilization of its 5'UTR and suppression of premature transcriptional termi-nation,but not by an increased transcription rate.Mapping of RNA 3'ends and in vitro cleavage assays revealed that RNase E cleaves within a stretch of six consecutive uridine residues within the rne 5'UTR,indicating autoregulation.These observations suggest that RNase E in cyanobacteria contributes to reshaping the transcriptome during the UV stress response and that its required activity level is secured at the RNA level despite the enhanced turnover of the protein.

cyanobacteriaprotein turnoverribonucleasestress response

Satoru Watanabe、Damir Stazic、Jens Georg、Shota Ohtake、Yutaka Sakamaki、Megumi Numakura、Munehiko Asayama、Taku Chibazakura、Annegret Wilde、Claudia Steglich、Wolfgang R.Hess

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Faculty of Biology,Genetics and Experimental Bioinformatics,University of Freiburg,Freiburg,Germany

Department of Bioscience,Tokyo University of Agriculture,Setagaya-ku,Tokyo,Japan

Nexxiot,Prime Tower(Hardstrasse 201),Zurich,Switzerland

School of Agriculture,Molecular Genetics,Ibaraki University,Ibaraki,Japan

Faculty of Biology,Molecular Genetics,University of Freiburg,Freiburg,Germany

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Ministry of Education,Culture,Sports,Science and Technology of JapanMinistry of Education,Culture,Sports,Science and Technology of JapanMinistry of Education,Culture,Sports,Science and Technology of JapanAdvanced Low Carbon Technology Research and Development Program(ALCA)of the Japan Science and Technology Agency(JST)German Ministry of Education and Research(BMBF)German Science Foundation(DFG)research training group MelnBioGerman Science Foundation(DFG)research training group MelnBio

Grantsin-Aid 25850056S230620K05793031L0164BRNAProNet322977937/GRK2344STE 1119/4-2

2023

10.1002/mlf2.12056

微生物(英文)

微生物(英文)

ISSN：
年,卷(期)：2023.2(1)
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