Engineering Nannochloropsis oceanica for the production of diterpenoid compounds

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外文摘要：Photosynthetic microalgae like Nannochloropsis hold enormous potential as sustainable,light-driven biofactories for the pro-duction of high-value natural products such as terpenoids.Nannochloropsis oceanica is distinguished as a particularly robust host with extensive genomic and transgenic resources available.Its capacity to grow in wastewater,brackish,and sea waters,coupled with advances in microalgal metabolic engineering,genome editing,and synthetic biology,provides an excellent opportunity.In the present work,we demonstrate how N.oceanica can be engineered to produce the diterpene casbene-an important intermediate in the biosynthesis of pharmacologically relevant macrocyclic diterpenoids.Casbene accumulated after stably expressing and targeting the casbene synthase from Daphne genkwa(DgTPS1)to the algal chloroplast.The engineered strains yielded production titers of up to 0.12 mg g-1 total dry cell weight(DCW)casbene.Heterologous overexpression and chloroplast targeting of two upstream rate-limiting enzymes in the 2-C-methyl-D-erythritol 4-phosphate pathway,Coleus forskohlii 1-deoxy-D-xylulose-5-phosphate synthase and geranylgeranyl diphosphate synthase genes,further enhanced the yield of casbene to a titer up to 1.80 mg g-1 DCW.The results presented here form a basis for further development and production of complex plant diterpenoids in microalgae.

外文关键词：

casbenemicroalgaeplastid targetingsynthetic biologyterpene synthase

作者：

Zhi-Yan Du、Wajid W.Bhat、Eric Poliner、Sean Johnson、Conor Bertucci、Eva Farre、Bjoern Hamberger

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作者单位：

Department of Molecular Biosciences and Bioengineering,University of Hawaii at Manoa,Honolulu,Hawaii,USA

Department of Biochemistry and Molecular Biology,Michigan State University,East Lansing,Michigan,USA

Department of Plant Biology,Michigan State University,East Lansing,Michigan,USA

New England Biolabs Inc.,240 County Roadlpswich,01938 MA,USA

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基金：

U.S.Department of Agriculture National Institute of Food and Agriculture(HATCH project )U.S.Department of Agriculture National Institute of Food and Agriculture(HATCH project )Center for Tropical and Subtropical AquacultureNational Science Foundationfacilities at the UH ManoaMichigan State UniversityMichigan State University Strategic Partnership Grant programUS Department of Energy-Great Lakes Bioenergy Research Center Cooperative Agreementstartup funding from the Department of Molecular Biology and BiochemistryUSDA National Institute of Food and Agriculture,HATCH projectNational Science FoundationNational Science Foundation

项目编号：

HAW05047-H2023-70003-387832020-38500-325592121410DE-SC0018409MICL024541737898IOS-134721

出版年：

2023

DOI：

10.1002/mlf2.12097

微生物(英文)

ISSN：

年,卷(期)：2023.2(4)

参考文献量76