Objective Preliminary study on the mechanism of baicalein against carbapenem-resistant Klebsiella pneumoniae by RNA sequencing(RNA-Seq).Methods Identification and drug sensitivity testing of strain T3 were conducted using VITEK MS mass spectrometer and Vitek2 Compact drug sensitivity analysis.The minimum inhibitory concentration of baica-lin against carbapenem-resistant Klebsiella pneumoniae T3 was determined by micro broth dilution method.RNA-Seq was performed on Klebsiella pneumoniae T3 treated with 1/2 MIC baicalein and normal control group by using the transcriptome sequencing platform Illumina NovaSeq 6000.Bioinformatics tools such as Trimmomatic,Rockhoppe,edgeR and Goatools were used to process the transcriptome data,and the obtained expression profiles were analyzed by differential expression,GO annotation,GO enrichment and KEGG metabolic pathway.Results Strain T3 is carbapenem-resistant Klebsiella pneumoni-ae,the MIC of baicalin against Klebsiella pneumoniae T3 was 160 μg/mL.Compared with the control group,the baicalin group had 723 differentially expressed genes,of which 659 were upreg-ulated and 64 were downregulated;differential gene GO annota-tion mainly involves 14 functional subclasses of biological processes,3 functional subclasses of cell component,and 10 func-tional subclasses of molecular functions;GO enrichment mainly occurs in regulation of biological quality,secondary metabol-ic process,etc;KEGG metabolic pathway is mainly enriched in phosphonate and phosphinate metabolism,biosynthesis of siderophore group nonribosomal peptides,ABC transporters,etc.Conclusion Baicalein inhibits the growth of carbapenem-resistant Klebsiella pneumoniae T3 through multiple ways,among which the main targets are regulation of biological quality,secondary metabolic process,phosphonate and phosphinate metabolism,biosynthesis of siderophore group nonribosomal pep-tides,ABC transporters,etc,which provides a basis for further research on baicalein against carbapenem-resistant Klebsiella pneumoniae.
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