Virulence genes and antimicrobial resistance of Bacillus cereus in prepackaged pastries sold in Taizhou from 2020 to 2022
OBJECTIVE To investigate the virulence genes and antimicrobial resistance of Bacillus cereus from the pre-packaged pastries in Taizhou city.METHODS 500 pre-packaged patries were collected in taizhou city market.97 Bacillus cereus strains were detected from them by GB 4789.14-2014 method and identified with 4 houseking genes,then 13 virulence genes were detected by polymerase chain reaction(PCR)method and the antimicrobial resistance of Bacillus cereus to 19 antibiotics was detected by paper diffusion method.RESULTS The result showed that the contamination rate of Bacillus cereus was 19.4％in 500 pre-packaged pastries.The detection rate of four housekeeping genes groEL,gyr B,rpoB and Vrr were 100％,94.8％,97.9％and 96.9％,respectively,and 89.7％at the same time.The virulence gene test result showed that the detection rate of nheABC,entFM,bceT,cytK and hblABCD were 91.8％,88.7％,61.9％,51.6％and 25.8％,emetic virulence genes had the lowest detection rate,ces and EMl were 4.1％,cer was 5.2％.97 Bacillus cereus strains show different degrees of drug resistance to 14 antimicrobials,the resistance rates to penicillin,ampicillin,cefotaxime and cotrimoxazole were higher than 95％,but they were completely sensitive to streptomycin,vancomycin and chloramphenicol.CONCLUSION There is a risk of contamination by diarrhea-type Bacillus cereus and vomiting-type Bacillus cereus in prepackaged pastries in Taizhou.The isolated and identified Bacillus cereus has multiple-drug resistance.
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