Protective effects of galacto-oligosaccharide and polydextrose on Caco-2 intestinal cell barrier injury model
OBJECTIVE To explore the protective effect of different ratios of galactose oligosaccharide(GOS)and polydextrose(PDX)on intestinal cell barrier damage model of Caco-2.METHODS The same batch of Caco-2 cells were cultured to form a cell barrier model and randomly divided into damaged model group without calcium,calcium-containing blank control group(1.8 mmol/L Ca2+),low-ratio/low-dose group(1.8 mmol/L Ca2++2mg/mL GOS+2 mg/mL PDX)and low-ratio/medium-dose group(1.8 mmol/L Ca2++4mg/mL GOS+4 mg/mL PDX),low-ratio/high-dose group(1.8 mmol/L Ca2++8 mg/mL GOS+8 mg/mL PDX)and high-ratio/low-dose group(1.8 mmol/L Ca2++0.8 mg/mL GOS+3.2 mg/mL PDX),high-ratio/medium-dose group(1.8 mmol/L Ca2++1.6 mg/mL GOS+6.4 mg/mL PDX),high-ratio/high-dose group(1.8 mmol/L Ca2++3.2 mg/mL GOS+12.8 mg/mL PDX),a total of 8 groups,three parallel groups were performed in each group.The Trans Epithelial Electrical Resistance value and apparent permeability coefficient value of each group were determined after 4 d culture,and the morphology of tight junction proteins ZO-1,Occludin and Claudin-1 were observed by immunofluorescence method,and the expression levels of inflammatory related factors in each group were determined by protein microarray method.RESULTS Compared with damaged model group,TEER ratio in calcium-containing blank control group was significantly increased(P＜0.05),while Papp value was significantly decreased(P＜0.05);Compared with calcium-containing blank control group,TEER ratio in low-ratio/medium-dose group and high-ratio/high-dose group was significantly increased(P＜0.05)while Papp value was significantly decreased(P＜0.05),and they could significantly down-regulate some inflammatory response related cytokines.The cell barrier was intact in all groups except for the compact junction protein structure in the model group.CONCLUSION Compared with Ca2+alone,the combination of two prebiotics can enhance the density of Caco-2 cell barrier and reduced the permeability of cell bypass.And it can significantly reduce the expression level of some inflammatory cytokines and effectively protect the intestinal cell barrier.
intestinal cell barrierCaco-2 cellsgalacto-oligosaccharidepolydextrosetight-junction protein
NETL
NSTL
万方数据
维普
