Effects of fine particulate matter on bone mass and osteogenic differentiation of bone marrow mesenchymal stem cells in mice
OBJECTIVE To observe the effects of exposure to fine particulate matter(PM2.5)on bone mass,microstructure,biomechanical properties,and osteogenic differentiation ability of bone marrow mesenchymal stem cells(BMSCs)in mice.METHODS,A total of 16 C57BL/6J mice aged 8 weeks were randomly divided into control group(NS group)and PM2.5 exposure group(PM group).NS group was given normal saline,PM group was given 14 mg/kg PM2.5 suspension,50 μL,poisoning every 3 day.After 10 weeks,the lungs of mice were taken for HE staining,and the left tibia was taken for Micro CT detection to analyze parameters related to cancellous and cortical bone.The right tibia was taken for HE staining to observe changes in bone trabeculae.Immunohistochemical staining was used to detect type Ⅰ collagen(Col Ⅰ),osteoprotegerin(OPG),and nuclear factor-κB receptor activating factor ligand(RANKL)protein expression,tartrate resistant acid phosphatase(TRAP)staining for detection of osteoclasts.Extract primary BMSCs from bilateral femurs,induce osteogenesis,and then perform alkaline phosphatase(ALP)staining to detect ALP activity,alizarin red staining to detect bone mineralization ability,real-time PCR to detect osteocalcin(OCN),ALP,OPG,and RANKL mRNA expression,and biomechanical testing to test the mechanical properties of the femur.RESULTS Compared with the NS group,the pulmonary alveolar structure of the PM group mice was disrupted and a large number of inflammatory cells gathered.Prompt for successful PM2.5 poisoning operation.Micro CT result showed that the bone mineral density(BMD)and bone volume fraction(BV/TV)of the PM group mice were 276.959±15.152 mg/cm3 and 0.208％±0.009％,respectively.The NS group had 316.709±28.205 mg/cm3 and 0.236％±0.019％,respectively.The PM group was lower than the NS group(P＜0.05),but the trabecular number(Tb.N)There was no statistically significant difference in parameters such as trabecular thickness(Tb.Th)and trabecular separation(Tb.SP)(P＞0.05).The HE staining result of the tibia showed that the trabeculae in the NS group were thick,dense,and uniform.The bone trabeculae in the PM group were slender,with a decrease in number,widened spacing,and sparse arrangement.The expression of Col Ⅰ(0.023±0.009)and OPG(0.036±0.010)in the PM group increased compared to the NS group(0.079±0.007,0.059±0.012),while the expression of RANKL(0.036±0.006)decreased compared to the NS group(0.022± 0.002)(P＜O.05);The number of TRAP positive particles increased in the PM group.The experimental result after osteoinduction of BMSCs in mice showed that compared with the NS group,the PM group had a decrease in the number of ALP positive cells and a decrease in the number of calcium nodules.The relative expression of ALP,OCN,and OPG mRNA in the PM group(0.375±0.021,0.585±0.088,0.768±0.112)was significantly reduced compared to the NS group(1.001±0.043,1.006±0.132,1.002± 0.086),while the relative expression of RANKL mRNA(1.278±0.118)was increased compared to the NS group(1.001±0.057)(P＜0.05).The biomechanical experimental result showed that the maximum deflection of the NS group was 0.337±0.031 mm,while the maximum deflection of the PM group was 0.258±0.041 mm.Compared with the NS group,the maximum deflection of the PM group decreased significantly(P＜0.05),and the maximum stress and maximum load showed a decreasing trend,but the difference was not statistically significant(P＞0.05).CONCLUSION After 10 weeks of exposure to PM2.5,it can affect the bone health of mice,and its mechanism may be related to increased osteoclast activity and inhibition of the osteogenic differentiation ability of BMSCs.
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NSTL
万方数据
维普
