SEC16B、DNAJC27、FTO和MC4R基因多态性与汉族学龄前儿童超重肥胖的关联性
Association of polymorphisms in SEC16B,DNAJC27,FTO and MC4R genes with overweight and obesity in Han preschool children
彭锣兰 1宫照龙 1韩超 1陈梁凯 2石丽丽 1赵金鹏 1王梦瑶 1陈晨 1卓勤 1霍军生 1李岩1
作者信息
- 1. 中国疾病预防控制中心营养与健康所,国家卫生健康委微量元素与营养重点实验室,北京 100050
- 2. 华中科技大学公共卫生学院营养与食品卫生学系,湖北省食品营养与安全重点实验室,武汉 430030;华中科技大学同济医学院公共卫生学院环境与健康教育部重点实验室,武汉 430030
- 折叠
摘要
目的 研究SEC16B基因rs633715、DNAJC27基因rs713586、FTO 基因rs11642015和MC4R基因rs6567160多态性与汉族学龄前儿童超重肥胖的关联性.方法 选取2022年来自"早期儿童营养包干预长期营养健康作用评估"项 目监测点的河南省和贵州省749名2～6岁汉族儿童为研究对象,分成超重肥胖组和正常对照组.采用 KASP SNP 分型技术对 rs633715、rs713586、rs1 1642015 和 rs6567160 进行基因多态性检测.使用x2检验分析4个位点的基因型多态性分布,使用多因素Logistic回归模型分析4个位点与学龄前儿童超重肥胖的关联性.结果 rs633715和rs6567160的基因型多态性在河南省和贵州省学龄前儿童中的分布有统计学意义(P＜0.05).rs633715位点的CC纯合突变型和隐性模型与学龄前儿童患超重肥胖的风险有关[OR及 95％CI 分别为 2.915(1.163～7.305)和 2.997(1.226～7.323),P＜0.05];rs713586位点的TC杂合突变型和显性模型与学龄前儿童患超重肥胖的风险有关[OR 及 95％CI 分别为 2.362(1.054～5.289)和 2.362(1.054～5.289),P＜0.05].rs1 1642015和rs6567160位点的多态性与学龄前儿童超重肥胖的关联无统计学意义.rs633715和rs713586的累积效应分析结果显示,携带风险基因型的数量与学龄前儿童患超重肥胖的风险存在正向关联(P趋势＜0.01).结论 在汉族学龄前儿童中,SEC16B基因rs633715和DNAJC27基因rs713586位点的多态性与超重肥胖易感性有关,且SEC16B基因rs633715和DNAJC27基因rs713586位点的多态性对学龄前儿童超重肥胖易感性有累积效应,携带风险基因型的数量与儿童超重肥胖风险呈正向关联.
Abstract
OBJECTIVE To investigate the association of polymorphisms in SEC16B rs633715,DNAJC27 rs713586,FTO rs11642015 and MC4R rs6567160 with overweight and obesity in Han Chinese preschool children.METHODS A total of 749 Han Chinese preschool children from Henan and Guizhou Province of Long-term Health Effects Assessment Project of Infants and Toddlers Nutritional Pack were selected for the study and divided into an overweight and obese group and a normal control group in 2022.rs633715,rs713586,rs11642015 and rs6567160 were genotyped using Kompetitive allele-specific PCR(KASP)technology.The distribution of genotypic polymorphisms was compared using the x2 test.The association between the four loci and overweight and obesity in preschool children was analyzed using a multifactorial logistic regression model.RESULTS The statistical analysis revealed a significant disparity(P＜0.05)in the distribution of genotypic polymorphisms of rs633715 and rs6567160 among preschoolers in Henan and Guizhou Province.CC heterozygous mutant and recessive models at rs633715 locus were associated with susceptibility to overweight and obesity in preschool children[OR and 95％CI 2.915(1.163-7.305),and 2.997(1.226-7.323),respectively,both P＜0.05].TC heterozygous mutant and dominant models at rs713586 locus were also associated susceptibility to overweight and obesity in preschool children(OR and 95％CI were 2.362(1.054-5.289)and 2.362(1.054-5.289),respectively,both P＜0.05).rs11642015 and rs6567160 loci were not associated with susceptibility to overweight and obesity in preschool children(P＞0.05).The result of the analysis of the cumulative effect of rs633715 and rs713586 showed that the number of genotypes carrying the risk genotype was positively associated with the risk of overweight and obesity in preschool children(Ptrend＜0.01).CONCLUSION Among Han Chinese preschool children,SEC16B rs633715 and DNAJC27 rs713586 were associated with susceptibility to overweight and obesity in preschool children.Moreover,rs633715 and rs713586 had a cumulative effect on susceptibility to overweight and obesity in preschool children,the number of risk genotypes carried was positively associated with childhood overweight and obesity risk.
关键词
SEC16B/DNAJC27/FTO/MC4R/基因多态性/超重肥胖Key words
SEC16B/DNAJC27/FTO/MC4R/genetic polymorphism/overweight and obesity引用本文复制引用
基金项目
国家重点研发计划(2020YFC2006301)
早期儿童营养包干预长期营养健康作用评估项目(131031107000200001)
出版年
2024
NETL
NSTL
万方数据