Simultaneous determination of six benzodiazepine sedatives residue in aquatic products by high performance liquid chromatography-triple quadrupole mass spectrometry
OBJECTIVE To establish a method for the simultaneous determination of 6 benzodiazepine sedatives residue in aquatic products by high performance liquid chromatography-triple quadrupole mass spectrometry.METHODS The samples were extracted with acetonitrile and purified by C18 solid phase extraction column.The sample solution was separated by Waters ACQUITY UPLC BEH C18 column(2.1 mm×50 mm,1.7 μm)using 0.1％formic acid and methanol as mobile phase for gradient elution,determined in multiple reaction monitoring mode and quantified by internal standard method.RESULTS Six benzodiazepine sedatives had a good linear relationship in the range of 1.0-50.0 µg/L with r＞0.9990,the limits of detection and limits of quantification were 0.3 and 1.0 pg/kg.Average recoveries for the analytes at 3 spiked levels ranged from 74.2％-108.0％with relative standard deviations of 1.1％-6.7％(n=6).CONCLUSION The method is simple,rapid,sensitive and accurate,which is suitable for simultaneous determination of 6 benzodiazepine sedatives residue in aquatic products.
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