Determination of twelve halobenzoquinones in drinking water by solid phase extraction-ultra-performance liquid chromatography-tandem mass spectrometry
OBJECTIVE To establish a method for twelve halobenzoquinones(HBQs)in drinking water by solid phase extraction-ultra-performance liquid chromatography coupled with electrospray-tandem mass spectrometry(SPE-UPLC-MS/MS).METHODS The drinking water was acidified with formic acid and concentrated by Bond Elut Plexa solid phase extraction column.The sample solution was separated using Waters ACQUITY HSS T3 column(100 mm × 2.1 mm,1.8 μm)with gradient elution using methanol-water containing 0.1％formic acid as mobile phase.The target compouds were detected in negtive electrospray ionization(ESI-)and multiple reaction monitoring.RESULTS The concentration of twelve HBQs showed good linearity in the range 5.0-150.0 ng/mL,respectively,with the correlation coefficients greater than 0.999.The limits of detection(LOD)of twelve HBQs were lower than 2.0 ng/mL,and the limits of quantification(LOQ)for twelve HBQs were lower than 5.0 ng/mL,respectively.The recoveries of three levels in the matrix were 70.0％-84.0％.The matrix effffect was 0.08-0.64.CONCLUSION The SPE-UPLC-MS/MS method has high sensitivity,good accuracy and fast analysis speed for the detection of halobenzoquinones in drinking water.
solid phase extraction-ultra-performance liquid chromatography coupled with electrospray-tandem mass spectrometrydrinking waterdisinfection byproductshalobenzoquinones
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