Tuoji Island,serving as an important part of the Miaodao islands,is a key point of marine transport routes connecting Jiaodong Peninsula and Liaodong Peninsula.Significant changes of cultural features and social organization have taken placed from Longshan period to Yueshi period in Haidai region,typically the inland areas.However,the subsistence strategy of individuals in marine island has rarely been evaluated due to the location far from the inland.In this paper,Carbon and Nitrogen stable isotope analysis of bone collagen from 18 individuals have been performed to reveal the dietary variation of Dakou site,Tuoji Island.Major of the bone collagen has elevated δ13C and δ15N,indicating a large amount of C4-based marine food or terrestrial animal protein consumption,whereas M4 and M18 have relatively depleted carbon isotopic data caused by more negative 813C food.According to the archaeological backgrounds,we propose the Dakou persons intensively relied on the millet agriculture and highly exploiting marine resources.Further compared with other published isotopic data in the same region during different periods,we suggest the intensive millet agriculture has consistently shaped the cultural development of the island area,even surrounded by the ocean and far away from the mainland.